MacIntyre E A, Earnhart C G, Kaattari S L
Department of Environmental and Aquatic Animal Health, School of Marine Science, Virginia Institute of Marine Science, College of William and Mary, Gloucester Point, VA 23062-1,316, USA.
Parasitology. 2003 Apr;126(Pt 4):293-302. doi: 10.1017/s003118200200286x.
Perkinsus marinus is responsible for a chronic disease (Dermo) of the Eastern oyster, Crassostrea virginica. In order to simulate the in vivo environment more closely, a chemically defined medium (JL-ODRP-3) was supplemented with tissue homogenate extracts or plasma from oysters possessing varying degrees of susceptibility to P. marinus infection. In media supplemented with extracts from highly susceptible oysters (C. virginica), P. marinus cells secreted elevated amounts of a set of low molecular weight serine proteases (LMP: 30-45 kDa) as assessed by enhanced digestion within gelatin-substrate SDS-PAGE gels. Oyster species of low susceptibility (C. gigas and C. ariakensis) did not exhibit this ability to upregulate P. marinus LMP expression. Oyster extract supplementation also led to pronounced changes in P. marinus cellular morphology, such that the cells were comparable to those observed within naturally infected oysters.
马里努斯派金虫是导致美国东海岸牡蛎（弗吉尼亚巨蛎）患慢性病（Dermo病）的病原体。为了更贴近地模拟体内环境，在化学成分明确的培养基（JL - ODRP - 3）中添加了来自对马里努斯派金虫感染具有不同易感性的牡蛎的组织匀浆提取物或血浆。在添加了高度易感牡蛎（弗吉尼亚巨蛎）提取物的培养基中，通过明胶底物SDS - PAGE凝胶内消化增强评估发现，马里努斯派金虫细胞分泌出大量一组低分子量丝氨酸蛋白酶（LMP：30 - 45 kDa）。低易感性的牡蛎种类（太平洋牡蛎和香港牡蛎）没有表现出上调马里努斯派金虫LMP表达的这种能力。添加牡蛎提取物还导致马里努斯派金虫细胞形态发生显著变化，使得这些细胞与在自然感染牡蛎中观察到的细胞相似。
