Abdel-Wahab Mohamed H
Pharmacology and Toxicology Department, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt.
Pharmacol Res. 2003 Jun;47(6):509-15. doi: 10.1016/s1043-6618(03)00039-2.
Dibromoacetonitrile (DBAN) is known to be water disinfectant by-product. Its broad-spectrum toxicity in different test systems in vivo and in vitro has been reported. Oxidative damage induced by DBAN may be partially responsible for its toxicity. Herein, the ability of DBAN to induce oxidative stress in mouse testis and possible protective effect of an antioxidant tertiary butylhydroquinone (TBHQ) were addressed. Male albino mice were injected with a single dose of DBAN (50mg/kg i.p.), and killed after 3h of treatment. Control animals received 10ml/kg body weight i.p. of the vehicle DMSO. In both experiments, cauda epididymis were dissected and sperm count and motility were investigated. Also, testicular activity of lactic dehydrogenase-x (LDH-x) isozyme and histopathological changes were examined. Furthermore, testicular content of malonyldialdehyde (MDA) and reduced glutathione (GSH) were determined. A single i.p. dose of DBAN caused decrease in sperm count and motility to approximately 88 and 84%, respectively, compared with control animals. A 46% decrease in testicular activity of LDH-x, compared with control animals, was observed. A significant accumulation of MDA in DBAN-treated animals was increased to 99% while testicular content of GSH was decreased by 56% compared to control animals. Compared to DBAN-treated animals, treatment with TBHQ (100mg/kg p.o.) prior exposure to DBAN showed a remarkable degree of protection as indicated by enhancement of sperm count and motility, testicular activity of LDH-x, and GSH. Accumulation of testicular content of MDA significantly decreased following TBHQ treatment compared to DBAN-treated animals. In conclusion, results presented here indicate that DBAN is capable to induce oxidative stress in mouse testis. TBHQ may play a protective role against DBAN-induced testicular cellular damage.
二溴乙腈（DBAN）是一种已知的水消毒副产物。其在体内和体外不同测试系统中的广谱毒性已有报道。DBAN诱导的氧化损伤可能部分导致其毒性。在此，研究了DBAN诱导小鼠睾丸氧化应激的能力以及抗氧化剂叔丁基对苯二酚（TBHQ）可能的保护作用。雄性白化小鼠腹腔注射单剂量DBAN（50mg/kg），处理3小时后处死。对照动物腹腔注射10ml/kg体重的溶剂二甲基亚砜（DMSO）。在两个实验中，均解剖附睾尾并研究精子数量和活力。此外，检测乳酸脱氢酶-x（LDH-x）同工酶的睾丸活性和组织病理学变化。此外，测定睾丸中丙二醛（MDA）和还原型谷胱甘肽（GSH）的含量。与对照动物相比，腹腔注射单剂量DBAN导致精子数量和活力分别下降至约88%和84%。与对照动物相比，观察到LDH-x的睾丸活性下降了46%。与对照动物相比，DBAN处理动物中MDA显著积累增加至99%，而睾丸中GSH含量下降了56%。与DBAN处理动物相比，在暴露于DBAN之前用TBHQ（100mg/kg口服）处理显示出显著的保护程度，表现为精子数量和活力、LDH-x的睾丸活性以及GSH的增强。与DBAN处理动物相比，TBHQ处理后睾丸中MDA含量的积累显著降低。总之，此处呈现的结果表明DBAN能够在小鼠睾丸中诱导氧化应激。TBHQ可能对DBAN诱导的睾丸细胞损伤起到保护作用。
