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孕期晚期暴露于ω-9脂肪酸会导致大鼠精子DNA片段化和氧化应激，进而损害精子活力和存活能力。

DNA fragmentation and oxidative stress compromise sperm motility and survival in late pregnancy exposure to omega-9 fatty acid in rats.

作者信息

Oluwakemi Oyelowo, Olufeyisipe Adegoke

机构信息

Department of Physiology, College of Medicine of the University of Lagos, Lagos, Nigeria.

出版信息

Iran J Basic Med Sci. 2016 May;19(5):511-20.

PMID:27403258
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4923472/
Abstract

OBJECTIVES

The aim of this study was to evaluate the oxidative status and DNA integrity in testes of wistar rat offspring exposed to omega-9 monounsaturated (MUFA) at different times of late organogenesis.

MATERIALS AND METHODS

Sixty female rats were divided into six groups of 10 animals. The first group served as control and received the drug vehicle, olive oil (1 ml/kg/day). The second, third, fourth, fifth and sixth group received 1000 mg/kg of oleic acid on gestation day 15 (D15), 16 (D16), 17 (D17), 18 (D18) and 19 (D19), respectively. Male pups were allowed to attain puberty and thereafter, blood was taken for hormonal analyses. Sperm count and motility were assessed. Testes homogenate was used for the determination of biochemical variables. Testes DNA was also determined.

RESULTS

The results showed that sperm count and motility were significantly decreased in the treated groups as compared to the control. There was a marked increase in the malondialdehyde level in rat testes from all of the treated groups as compared to the control (P<0.05). DNA from the testes of rats of D19 had the highest level of fragmentation as compared to the control.

CONCLUSION

Omega-9 MUFA exposure in utero imposes negative effects on sperm variables and increases the level of sperm DNA fragmentation and oxidative stress.

摘要

目的

本研究旨在评估在器官发育后期不同时间暴露于ω-9单不饱和脂肪酸（MUFA）的Wistar大鼠后代睾丸中的氧化状态和DNA完整性。

材料与方法

60只雌性大鼠分为6组，每组10只。第一组作为对照组，给予药物载体橄榄油（1毫升/千克/天）。第二、三、四、五和六组分别在妊娠第15天（D15）、16天（D16）、17天（D17）、18天（D18）和19天（D19）接受1000毫克/千克的油酸。雄性幼崽进入青春期后，采集血液进行激素分析。评估精子数量和活力。睾丸匀浆用于测定生化变量。还测定了睾丸DNA。

结果

结果显示，与对照组相比，处理组的精子数量和活力显著降低。与对照组相比，所有处理组大鼠睾丸中的丙二醛水平均显著升高（P<0.05）。与对照组相比，D19组大鼠睾丸DNA的片段化水平最高。

结论

子宫内暴露于ω-9 MUFA会对精子变量产生负面影响，并增加精子DNA片段化水平和氧化应激。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c781/4923472/47f780e0120a/IJBMS-19-511-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c781/4923472/1725c87d80ab/IJBMS-19-511-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c781/4923472/4cc16e9b06b1/IJBMS-19-511-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c781/4923472/39b6e7e0eb9e/IJBMS-19-511-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c781/4923472/f4ddab28cea0/IJBMS-19-511-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c781/4923472/10515ff803df/IJBMS-19-511-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c781/4923472/124411fc6a4f/IJBMS-19-511-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c781/4923472/72af2a134b88/IJBMS-19-511-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c781/4923472/e2db8d9ec908/IJBMS-19-511-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c781/4923472/47f780e0120a/IJBMS-19-511-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c781/4923472/1725c87d80ab/IJBMS-19-511-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c781/4923472/4cc16e9b06b1/IJBMS-19-511-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c781/4923472/39b6e7e0eb9e/IJBMS-19-511-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c781/4923472/f4ddab28cea0/IJBMS-19-511-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c781/4923472/10515ff803df/IJBMS-19-511-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c781/4923472/124411fc6a4f/IJBMS-19-511-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c781/4923472/72af2a134b88/IJBMS-19-511-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c781/4923472/e2db8d9ec908/IJBMS-19-511-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c781/4923472/47f780e0120a/IJBMS-19-511-g009.jpg

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