脑心肌炎病毒的无细胞合成
Cell-free synthesis of encephalomyocarditis virus.
作者信息
Svitkin Yuri V, Sonenberg Nahum
机构信息
Department of Biochemistry and McGill Cancer Center, McGill University, Montreal, Quebec, Canada H3G 1Y6.
出版信息
J Virol. 2003 Jun;77(11):6551-5. doi: 10.1128/jvi.77.11.6551-6555.2003.
Abstract
We developed a system for complete replication of encephalomyocarditis virus (EMCV) in a test tube by using an in vitro translation extract from Krebs-2 cells. Efficient virus synthesis occurred in a narrow range of Mg(2+) and EMCV RNA concentrations. Excess input RNA impaired RNA replication and virus production but not translation. This suggests the existence of a negative-feedback mechanism for regulation of RNA replication by the viral plus-strand RNA or proteins.
摘要
我们利用克雷布斯2细胞的体外翻译提取物，开发了一种在试管中完全复制脑心肌炎病毒（EMCV）的系统。在狭窄的镁离子（Mg(2+)）和EMCV RNA浓度范围内发生了高效的病毒合成。过量输入的RNA会损害RNA复制和病毒产生，但不会影响翻译。这表明存在一种由病毒正链RNA或蛋白质调控RNA复制的负反馈机制。
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本文引用的文献
1
Poliovirus requires a precise 5' end for efficient positive-strand RNA synthesis.
J Virol. 2000 Jul;74(14):6394-400. doi: 10.1128/jvi.74.14.6394-6400.2000.
2
Switch from translation to RNA replication in a positive-stranded RNA virus.
Genes Dev. 1998 Aug 1;12(15):2293-304. doi: 10.1101/gad.12.15.2293.
3
Rapamycin and wortmannin enhance replication of a defective encephalomyocarditis virus.
J Virol. 1998 Jul;72(7):5811-9. doi: 10.1128/JVI.72.7.5811-5819.1998.
4
Assays for poliovirus polymerase, 3D(Pol), and authentic RNA replication in HeLa S10 extracts.
Methods Enzymol. 1996;275:35-57. doi: 10.1016/s0076-6879(96)75005-x.
5
Canonical eukaryotic initiation factors determine initiation of translation by internal ribosomal entry.
Mol Cell Biol. 1996 Dec;16(12):6859-69. doi: 10.1128/MCB.16.12.6859.
6
Effects of temperature and lipophilic agents on poliovirus formation and RNA synthesis in a cell-free system.
J Virol. 1993 Oct;67(10):5932-8. doi: 10.1128/JVI.67.10.5932-5938.1993.
7
Dominant negative mutants of mammalian translation initiation factor eIF-4A define a critical role for eIF-4F in cap-dependent and cap-independent initiation of translation.
EMBO J. 1994 Mar 1;13(5):1205-15. doi: 10.1002/j.1460-2075.1994.tb06370.x.
8
Complete replication of poliovirus in vitro: preinitiation RNA replication complexes require soluble cellular factors for the synthesis of VPg-linked RNA.
J Virol. 1995 Sep;69(9):5516-27. doi: 10.1128/JVI.69.9.5516-5527.1995.
9
Differences between translation products of tick-borne encephalitis virus RNA in cell-free systems from Krebs-2 cells and rabbit reticulocytes: involvement of membranes in the processing of nascent precursors of flavivirus structural proteins.
Virology. 1984 Jun;135(2):536-41. doi: 10.1016/0042-6822(84)90207-1.
10
Preparation of microsomal membranes for cotranslational protein translocation.
Methods Enzymol. 1983;96:84-93. doi: 10.1016/s0076-6879(83)96010-x.
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