经典的真核生物起始因子通过核糖体内部进入来决定翻译起始。
Canonical eukaryotic initiation factors determine initiation of translation by internal ribosomal entry.
作者信息
Pestova T V, Hellen C U, Shatsky I N
机构信息
Department of Microbiology and Immunology, Morse Institute for Molecular Genetics, State University of New York Health Science Center at Brooklyn, 11203-2098, USA.
出版信息
Mol Cell Biol. 1996 Dec;16(12):6859-69. doi: 10.1128/MCB.16.12.6859.
Abstract
Translation of picornavirus RNA is initiated after ribosomal binding to an internal ribosomal entry site (IRES) within the 5' untranslated region. We have reconstituted IRES-mediated initiation on encephalomyocarditis virus RNA from purified components and used primer extension analysis to confirm the fidelity of 48S preinitiation complex formation. Eukaryotic initiation factor 2 (eIF2), eIF3, and eIF4F were required for initiation; eIF4B and to a lesser extent the pyrimidine tract-binding protein stimulated this process. We show that eIF4F binds to the IRES in a novel cap-independent manner and suggest that cap- and IRES-dependent initiation mechanisms utilize different modes of interaction with this factor to promote ribosomal attachment to mRNA.
摘要
微小核糖核酸病毒RNA的翻译是在核糖体与5'非翻译区内的内部核糖体进入位点（IRES）结合后启动的。我们已从纯化成分中重建了脑心肌炎病毒RNA上IRES介导的起始过程，并使用引物延伸分析来确认48S起始前复合物形成的保真度。起始需要真核起始因子2（eIF2）、eIF3和eIF4F；eIF4B以及程度较轻的嘧啶序列结合蛋白刺激了这一过程。我们表明，eIF4F以一种新的不依赖帽的方式与IRES结合，并提出依赖帽和依赖IRES的起始机制利用与该因子不同的相互作用模式来促进核糖体与mRNA的附着。
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本文引用的文献
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Functional dissection of eukaryotic initiation factor 4F: the 4A subunit and the central domain of the 4G subunit are sufficient to mediate internal entry of 43S preinitiation complexes.真核生物起始因子4F的功能剖析：4A亚基和4G亚基的中央结构域足以介导43S起始前复合物的内部进入。
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Internal initiation of translation directed by the 5'-untranslated region of the mRNA for eIF4G, a factor involved in the picornavirus-induced switch from cap-dependent to internal initiation.由eIF4G的mRNA的5'-非翻译区指导的翻译内部起始，eIF4G是一种参与微小核糖核酸病毒诱导的从帽依赖性起始向内部起始转变的因子。
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