一种木二糖衍生的异福明内酰胺糖苷酶抑制剂以其酰胺互变异构体的形式结合。

A xylobiose-derived isofagomine lactam glycosidase inhibitor binds as its amide tautomer.

作者信息

Gloster Tracey, Williams Spencer J, Tarling Chris A, Roberts Shirley, Dupont Claude, Jodoin Pascalé, Shareck François, Withers Stephen G, Davies Gideon J

机构信息

Structural Biology Laboratory, Department of Chemistry, The University of York, Heslington, UK.

出版信息

Chem Commun (Camb). 2003 Apr 21(8):944-5. doi: 10.1039/b301829f.

DOI:10.1039/b301829f

PMID:12744311

Abstract

The atomic-resolution structure of a xylobiose-derived isofagomine lactam in complex with the xylanase Xyn10A from Streptomyces lividans reveals that the lactam is bound to the enzyme as the amide tautomer, with "reversed" protonation-states for nucleophile and acid-base.

摘要

来自淡紫链霉菌的木聚糖酶Xyn10A与木二糖衍生的异法戈明内酰胺的原子分辨率结构表明，内酰胺以酰胺互变异构体的形式与该酶结合，亲核试剂和酸碱的质子化状态“相反”。