木二糖衍生的脱氧野尻霉素和异法戈明与木聚糖酶Xyn10A结合的原子分辨率分析。

Atomic resolution analyses of the binding of xylobiose-derived deoxynojirimycin and isofagomine to xylanase Xyn10A.

作者信息

Gloster Tracey M, Williams Spencer J, Roberts Shirley, Tarling Chris A, Wicki Jacqueline, Withers Stephen G, Davies Gideon J

机构信息

Structural Biology Laboratory, Department of Chemistry, University of York, Heslington, UK.

出版信息

Chem Commun (Camb). 2004 Aug 21(16):1794-5. doi: 10.1039/b405152a. Epub 2004 Jul 23.

DOI:10.1039/b405152a

PMID:15306887

Abstract

The atomic resolution structures of xylobiose-derived isofagomine and xylobiose-derived deoxynojirimycin in complex with the xylanase Xyn10A from Streptomyces lividans reveal undistorted (4)C(1) chair conformed sugars and, in the case of the deoxynojirimycin analogue, suggest unusual pK(a) changes of the enzyme's catalytic machinery upon binding.

摘要

来源于木二糖的异麦角硫因和来源于木二糖的脱氧野尻霉素与来自淡紫链霉菌的木聚糖酶Xyn10A形成的复合物的原子分辨率结构显示糖呈未扭曲的（4）C（1）椅式构象，并且对于脱氧野尻霉素类似物而言，表明结合后酶催化机制的pK（a）发生异常变化。