de Oliveira Mendes Carolina, Paraguai de Souza Edilma, Borja-Cabrera Gulnara Patrícia, Maria Melo Batista Leopoldina, Aparecida dos Santos Miriam, Ellner Parra Luciana, Menz Ingrid, Palatnik Marcos, Palatnik de Sousa Clarisa Beatriz
Instituto de Microbiologia, "Prof Paulo de Góes", Universidade Federal do Rio de Janeiro (UFRJ), CCS, Cidade Universitária, Ilha do Fundão, CP 68040, CEP 21941-590, Rio de Janeiro, RJ, Brazil.
Vaccine. 2003 Jun 2;21(19-20):2589-97. doi: 10.1016/s0264-410x(03)00046-x.
Canine antibody IgG, IgG1 and IgG2 anti-FML responses were investigated in dogs vaccinated with the fucose-mannose ligand (FML)-vaccine of Leishmania donovani and in dogs with naturally acquired visceral leishmaniosis. While similar levels of total IgG antibodies were seen in the seropositive naturally infected dogs and in vaccinees, significant differences between the groups were found regarding their IgG1/IgG2 anti-FML antibody composition (P<0.005). Higher IgG1 absorbencies were seen in infected dogs, while the IgG2 subtype was predominant in pre-immune sera, and in vaccinated animals, both after the first and the third dose (P<0.005). The average ratio between IgG1/IgG2 was then 1.124 for infected animals and 0.733 for FML-vaccinees. Also, a significant increase in IgG2 antibodies was observed from the first to the third vaccine injection (P<0.005). In the infected dogs, a high correlation between their IgG absorbance (Abs) values and the number of symptoms (P=0.017) was disclosed. Thus, the analysis of IgG subclasses disclosed a dichotomous response to visceral leishmaniosis: IgG1 associated to natural infection and IgG2 associated to a humoral response subsequent to the FML-vaccine treatment. An IgG1/IgG2>or=1 would characterize the sera of visceral leishmaniasis infected animals evoluting towards the overt disease while ratios <or=1 would characterize the sera response of vaccinated protected dogs.
对接种杜氏利什曼原虫岩藻糖 - 甘露糖配体（FML）疫苗的犬以及自然感染内脏利什曼病的犬的犬抗体IgG、IgG1和IgG2抗FML反应进行了研究。虽然在血清阳性的自然感染犬和接种疫苗的犬中观察到总IgG抗体水平相似，但在IgG1/IgG2抗FML抗体组成方面发现两组之间存在显著差异（P<0.005）。感染犬中观察到较高的IgG1吸光度，而IgG2亚型在免疫前血清中占主导地位，在接种动物中，在第一剂和第三剂后也是如此（P<0.005）。然后，感染动物的IgG1/IgG2平均比率为1.124，FML疫苗接种动物为0.733。此外，从第一次疫苗注射到第三次疫苗注射观察到IgG2抗体显著增加（P<0.005）。在感染犬中，其IgG吸光度（Abs）值与症状数量之间存在高度相关性（P = 0.017）。因此，IgG亚类分析揭示了对内脏利什曼病的二分反应：IgG1与自然感染相关，IgG2与FML疫苗治疗后的体液反应相关。IgG1/IgG2>或 = 1将表征内脏利什曼病感染动物向显性疾病发展的血清，而比率<或 = 1将表征接种疫苗的受保护犬的血清反应。
