使用快速高效的液-液萃取和固相萃取法测定血浆中[18F]FCWAY、[18F]FP-TZTP及其代谢物。

Determination of [18F]FCWAY, [18F]FP-TZTP, and their metabolites in plasma using rapid and efficient liquid-liquid and solid phase extractions.

作者信息

Ma Ying, Kiesewetter Dale O, Lang Lixin, Der Margaret, Huang Bill, Carson Richard E, Eckelman William C

机构信息

PET Department, The Warren G Magnuson Clinical Center, National Institutes of Health, Bethesda, MD, USA.

出版信息

Nucl Med Biol. 2003 Apr;30(3):233-40. doi: 10.1016/s0969-8051(02)00452-3.

DOI:10.1016/s0969-8051(02)00452-3

PMID:12745014

Abstract

Liquid-liquid and solid phase extraction methods were developed for the accurate and rapid quantitation of radioactive components in human plasma following injection of two PET ligands. A solid phase extraction (SPE) method was developed for the determination of the 5HT(1A) receptor ligand [N-[2-[4-(2-methoxyphenyl) piperazino]ethyl]-N-(2-pyridinyl) trans-4-[(18)F]fluorocyclohexanecarboxamide (FCWAY), and its acidic metabolite, 4-[(18)F]fluorocyclohexane carboxylic acid (FC). In both cases, the extraction method was much faster and easier to use, yet provided results comparable to HPLC and TLC methods. In addition, an easy to perform two-step liquid-liquid extraction was developed for quantitation of 3-(3-((3-[(18)F]fluoropropyl)thio)-1,2,5-thiadiazol-4-yl)-1,2,5,6-tetrahydro-1-methylpyridine ([(18)F]FP-TZTP), a selective M2 muscarinic agonist.

摘要

开发了液-液萃取和固相萃取方法，用于在注射两种正电子发射断层显像（PET）配体后准确快速地定量人血浆中的放射性成分。开发了一种固相萃取（SPE）方法，用于测定5HT(1A)受体配体[N-[2-[4-(2-甲氧基苯基)哌嗪基]乙基]-N-(2-吡啶基)反式-4-[(18)F]氟环己烷甲酰胺（FCWAY）及其酸性代谢物4-[(18)F]氟环己烷羧酸（FC）。在这两种情况下，萃取方法更快且更易于使用，但其提供的结果与高效液相色谱（HPLC）和薄层色谱（TLC）方法相当。此外，还开发了一种易于操作的两步液-液萃取方法，用于定量3-(3-((3-[(18)F]氟丙基)硫代)-1,2,5-噻二唑-4-基)-1,2,5,6-四氢-1-甲基吡啶([(18)F]FP-TZTP)，一种选择性M2毒蕈碱激动剂。