Bigelow Chad E, Conover David L, Foster Thomas H
University of Rochester, Department of Radiology and the Institute of Optics, 601 Elmwood Avenue, Box 648, Rochester, New York 14642, USA.
Opt Lett. 2003 May 1;28(9):695-7. doi: 10.1364/ol.28.000695.
We report the design and implementation of a laser scanning confocal fluorescence system with spectroscopy and anisotropy imaging capabilities. Confocal spectroscopy is achieved with a fiber pinhole that is inserted into and removed from the detection path as needed. Fluorescence anisotropy imaging is accomplished with a polarizing beam splitter placed after the conventional pinhole. Two orthogonal polarizations are detected simultaneously with balanced photomultiplier tubes. The quality of the axial sectioning that is achieved in the confocal fluorescence spectroscopy mode is demonstrated experimentally, and examples of polarization-sensitive fluorescence imaging are demonstrated in tumor cell monolayers.
我们报告了一种具有光谱学和各向异性成像功能的激光扫描共聚焦荧光系统的设计与实现。通过一个根据需要插入和移出检测路径的光纤针孔实现共聚焦光谱学。荧光各向异性成像通过在传统针孔之后放置一个偏振分束器来完成。利用平衡光电倍增管同时检测两个正交偏振。实验证明了在共聚焦荧光光谱模式下实现的轴向切片质量，并展示了肿瘤细胞单层中偏振敏感荧光成像的实例。
