Johnson P K, Yoder J M, Adams E C
Am J Obstet Gynecol. 1976 May 1;125(1):45-50. doi: 10.1016/0002-9378(76)90889-9.
A rapid, flow-through, column, nonequilibrium radioimmunoassay for human chorionic somatomammotropin (HCS) is described. The total incubation time is about 1 hour, and no centrifugation is needed. Reproducibility, sensitivity, specificity, recovery, and parallelism are adequate for clinical utility. Hormone levels in a variety of pregnancy sera assayed by this column method are closely related to results from another more conventional radioimmunoassay method.
本文描述了一种用于人绒毛膜生长催乳素(HCS)的快速、流通式、柱式非平衡放射免疫测定法。总孵育时间约为1小时,无需离心。其重现性、灵敏度、特异性、回收率和平行性对于临床应用而言是足够的。用这种柱式方法测定的各种妊娠血清中的激素水平与另一种更传统的放射免疫测定法的结果密切相关。