Mori M
Acta Med Okayama. 1975 Jun;29(3):161-9.
A study was carried out to clarify the mechanism of nuclear extrusion of mammalian erythroid cells by observing erythroblasts of rabbit under various conditions in vitro. The animals were made anemic by phenylhydrazine injection and erythroblasts were obtained from the peripheral blood and observed morphologically after a certain time of incubation. After two hour incubation at 37 degrees C, about 50% of erythroblasts were denucleated. The nuclear extrusion was remarkably suppressed by the inhibitor for electron transport system or by uncouplers for oxidative phosphorylation. It was also arrested by the inhibitor of cell movement, like cytochalasin B. In contrast, monoiodo-acetic acid, ouabain and colchicine hardly inhibited the nuclear extrusion. The observations indicated that the nuclear extrusion of mammalian erythroblast is an energy-dependent process in connection with the function of contractile microfilamentous system susceptible to cytochalasin B.
通过在体外不同条件下观察兔成红细胞，开展了一项研究以阐明哺乳动物红细胞核排出的机制。通过注射苯肼使动物贫血，从外周血获取成红细胞，并在孵育一定时间后进行形态学观察。在37℃孵育两小时后，约50%的成红细胞去核。电子传递系统抑制剂或氧化磷酸化解偶联剂可显著抑制核排出。细胞运动抑制剂，如细胞松弛素B，也可阻止核排出。相比之下，一碘乙酸、哇巴因和秋水仙碱几乎不抑制核排出。这些观察结果表明，哺乳动物成红细胞的核排出是一个与易受细胞松弛素B影响的收缩性微丝系统功能相关的能量依赖性过程。
