González-Suárez Ignacio, Naves Manuel, Díaz-Corte Carmen, Fernández-Martín José L, Menéndez-Rodríguez Primitiva, Cannata-Andía Jorge B
Bone and Mineral Research Unit, Instituto Reina Sofía de Investigación, Hospital Central de Asturias, Oviedo, Spain.
Kidney Int Suppl. 2003 Jun(85):S39-43. doi: 10.1046/j.1523-1755.63.s85.10.x.
To assess the effect of aluminium on the calcium-sensing receptor expression, proliferation, and apoptosis in parathyroid glands from rats with chronic renal failure, 2(1/2)-month-old male Wistar rats were 7/8 nephrectomized.
Eight weeks after surgery the rats were divided into two groups, one receiving intraperitoneal AlCl3 for 8 weeks and the other receiving intraperitoneal placebo. Serum Al, Ca, P, creatinine, and PTH were measured. Parathyroid glands were removed, formaldehyde-fixed, and paraffin-embedded. Calcium-sensing receptor and proliferation were detected by immunohistochemistry and apoptosis by TUNEL and propidium iodide uptake.
At the end of the study, despite higher levels of serum P in the aluminium group (6.27 +/- 0.63 vs. 5.56 +/- 0.58 mg/dL; P = 0.045), serum PTH was lower (89.6 +/- 57.7 vs. 183.1 +/- 123.8 pg/mL; P = 0.059). No significant differences were found in the calcium-sensing receptor expression between groups (aluminium: 27.1 +/- 7.6; placebo: 25.4 +/- 3.5 RU). Rats receiving aluminium showed a significantly lower cell proliferation rate than the control rats (0.54 +/- 0.69 vs. 4.43 +/- 3.10 cells/mm2; P = 0.003). No apoptotic events were detected.
Aluminium was able to reduce the cell proliferation of the parathyroid glands. Due to the low apoptosis rate, however, it was not possible to find any change. Aluminium had no effect on the calcium-sensing receptor expression.
为评估铝对慢性肾衰竭大鼠甲状旁腺中钙敏感受体表达、细胞增殖及细胞凋亡的影响，将2（1/2）月龄雄性Wistar大鼠进行7/8肾切除。
术后8周将大鼠分为两组，一组腹腔注射氯化铝8周，另一组腹腔注射安慰剂。检测血清铝、钙、磷、肌酐及甲状旁腺激素水平。切除甲状旁腺，用甲醛固定，石蜡包埋。采用免疫组化法检测钙敏感受体及细胞增殖情况，用TUNEL法和碘化丙啶摄取法检测细胞凋亡情况。
研究结束时，尽管铝组血清磷水平较高（6.27±0.63 vs. 5.56±0.58 mg/dL；P = 0.045），但血清甲状旁腺激素水平较低（89.6±57.7 vs. 183.1±123.8 pg/mL；P = 0.059）。两组间钙敏感受体表达无显著差异（铝组：27.1±7.6；安慰剂组：25.4±3.5 RU）。接受铝处理的大鼠细胞增殖率显著低于对照大鼠（0.54±0.69 vs. 4.43±3.10细胞/mm²；P = 0.003）。未检测到凋亡事件。
铝能够降低甲状旁腺细胞的增殖。然而，由于凋亡率较低，未发现任何变化。铝对钙敏感受体表达无影响。
