卡勒红素的核磁共振溶液结构，一种来自糖多孢红霉菌的EF手型钙结合蛋白。

NMR solution structure of calerythrin, an EF-hand calcium-binding protein from Saccharopolyspora erythraea.

作者信息

Tossavainen Helena, Permi Perttu, Annila Arto, Kilpeläinen Ilkka, Drakenberg Torbjörn

机构信息

NMR laboratory, Structural Biology and Biophysics, Institute of Biotechnology, University of Helsinki, Finland.

出版信息

Eur J Biochem. 2003 Jun;270(11):2505-12. doi: 10.1046/j.1432-1033.2003.03623.x.

DOI:10.1046/j.1432-1033.2003.03623.x

PMID:12755706

Abstract

The structure of calerythrin, a prokaryotic 20 kDa calcium-binding protein has been determined by solution NMR spectroscopy. Distance, dihedral angle, J coupling, secondary chemical shift, residual dipolar coupling and radius of gyration restraints reveal four EF-hand motifs arranged in a compact globular structure. A tight turn in the middle of the amino acid sequence brings the two halves, each comprising a pair of EF-hands, close together. The structural similarity between calerythrin and the eukaryotic sarcoplasmic calcium-binding proteins is notable.

摘要

芹菜红素（一种原核生物20 kDa钙结合蛋白）的结构已通过溶液核磁共振光谱法测定。距离、二面角、J耦合、二级化学位移、剩余偶极耦合和回转半径限制揭示了四个EF手基序排列成紧密的球状结构。氨基酸序列中间的一个紧密转角使两半部分（每半部分包含一对EF手）靠近在一起。芹菜红素与真核肌浆钙结合蛋白之间的结构相似性很显著。