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大蒜衍生的有机硫成分阿霍烯通过诱导细胞凋亡来减小基底细胞癌的肿瘤大小。

The garlic-derived organosulfur component ajoene decreases basal cell carcinoma tumor size by inducing apoptosis.

作者信息

Tilli C M L J, Stavast-Kooy A J W, Vuerstaek J D D, Thissen M R T M, Krekels G A M, Ramaekers F C S, Neumann H A M

机构信息

Research Institute Growth and Development (GROW), University of Maastricht, The Netherlands.

出版信息

Arch Dermatol Res. 2003 Jul;295(3):117-23. doi: 10.1007/s00403-003-0404-9. Epub 2003 May 20.

Abstract

Although the therapeutic role of ajoene, an organosulfur compound of garlic, in cardiovascular diseases and mycology has been established, its usefulness in cancer treatment has only recently been suggested. We applied ajoene topically to the tumors of 21 patients with either nodular or superficial basal cell carcinoma (BCC). A reduction in tumor size was seen in 17 patients. Immunohistochemical assays for Bcl-2 expression in a selection of these tumors before and after treatment showed a significant decrease in this apoptosis-suppressing protein. On average, the percentage of tumor cells expressing the proliferation marker Ki-67 was not decreased, which suggests that the action of ajoene is not explained by a cytostatic effect. To obtain further insight into the mode of action of ajoene, the BCC cell line TE354T and a short-term primary culture of BCC were analyzed for apoptosis induction after treatment with the drug. Apoptosis was detected by morphology of the cells and by flow cytometry. Ajoene induced apoptosis in a dose- and time-dependent manner in these cultures. Taking together the results of the in vivo and in vitro studies, we conclude that ajoene can reduce BCC tumor size, mainly by inducing the mitochondria-dependent route of apoptosis.

摘要

尽管大蒜中的有机硫化合物阿霍烯在心血管疾病和真菌学方面的治疗作用已得到证实,但其在癌症治疗中的效用直到最近才被提出。我们对21例患有结节性或浅表性基底细胞癌(BCC)的患者的肿瘤局部应用了阿霍烯。17例患者的肿瘤大小有所减小。对这些肿瘤中的一部分在治疗前后进行Bcl-2表达的免疫组化分析显示,这种凋亡抑制蛋白显著减少。平均而言,表达增殖标志物Ki-67的肿瘤细胞百分比没有降低,这表明阿霍烯的作用不能用细胞抑制作用来解释。为了进一步深入了解阿霍烯的作用方式,在用该药物处理后,对BCC细胞系TE354T和BCC的短期原代培养物进行了凋亡诱导分析。通过细胞形态学和流式细胞术检测凋亡。阿霍烯在这些培养物中以剂量和时间依赖性方式诱导凋亡。综合体内和体外研究结果,我们得出结论,阿霍烯可主要通过诱导线粒体依赖性凋亡途径来减小BCC肿瘤大小。

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