Auswinporn Somsri, Jenwitheesuk Ekachai, Panburana Panyu, Sirinavin Sayomporn, Vibhagool Asda, Chantratita Wasun
Virology and Molecular Microbiology Unit, Department of Pathology, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
Southeast Asian J Trop Med Public Health. 2002 Dec;33(4):818-21.
HIV-1 drug resistance may limit the use of antiretrovirals when attempting to reduce the vertical transmission rate. Establishing the prevalence of the HIV-1 mutations associated with antiretroviral resistance in pregnant women will enable clinicians to maximize the chances of preventing vertical transmission. In order to determine the prevalence of HIV-1 resistant strains among antiretroviral-naive pregnant Thai women, the nucleotide sequences of the HIV-1 polymerase (pol) gene were evaluated. The plasma samples were collected from the women during the 34th week of pregnancy: numerous secondary mutations could be found in the reverse transcriptase (RT) and protease gene, while no primary mutations in the pol gene were found. The result also showed that by detecting the delta32bp deletion within the CCR 5 locus, it was evident that none of HIV-1 infected individuals had homozygous or heterozygous delta32bp deletions of the CCR5 gene; moreover, no CCR5 gene mutations were found in any individual.
在试图降低垂直传播率时，HIV-1耐药性可能会限制抗逆转录病毒药物的使用。确定与抗逆转录病毒耐药性相关的HIV-1突变在孕妇中的流行情况，将使临床医生能够最大限度地提高预防垂直传播的几率。为了确定未接受过抗逆转录病毒治疗的泰国孕妇中HIV-1耐药菌株的流行情况，对HIV-1聚合酶（pol）基因的核苷酸序列进行了评估。在妊娠第34周从这些女性身上采集血浆样本：在逆转录酶（RT）和蛋白酶基因中发现了许多二级突变，而在pol基因中未发现一级突变。结果还表明，通过检测CCR5基因座内的delta32bp缺失，很明显没有HIV-1感染个体具有CCR5基因的纯合或杂合delta32bp缺失；此外，在任何个体中均未发现CCR5基因突变。
