内皮细胞在激活和凋亡过程中释放出表型和数量上均不同的微粒。

Endothelial cells release phenotypically and quantitatively distinct microparticles in activation and apoptosis.

作者信息

Jimenez Joaquin J, Jy Wenche, Mauro Lucia M, Soderland Carl, Horstman Lawrence L, Ahn Yeon S

机构信息

Wallace H Coulter Platelet Laboratory, Division of Hematology/Oncology, University of Miami School of Medicine, 1600 NW 10th Ave, Mail Code R36A, Miami, FL, USA.

出版信息

Thromb Res. 2003 Feb 15;109(4):175-80. doi: 10.1016/s0049-3848(03)00064-1.

DOI:10.1016/s0049-3848(03)00064-1

PMID:12757771

Abstract

BACKGROUND

Endothelial cells (EC) shed endothelial microparticles (EMP) in activation and apoptosis.

OBJECTIVES

We compared the antigenic expression of EMP species released during activation as compared to apoptosis, in three cell lines.

METHODS

EC from renal and brain microvascular (MiVEC) and coronary macrovascular (MaVEC) origin were incubated with TNF-alpha to induce activation, or deprived of growth factors to induce apoptosis. Antigens expressed on EMP and EC were assayed flow cytometrically and included constitutive markers (CD31, CD51/61, CD105), inducible markers (CD54, CD62E and CD106), and annexin V binding.

RESULTS

It was found that in apoptosis, constitutive markers in EMP were markedly increased (CD31>CD105), with a concomitant decrease in expression in EC. Annexin V EC surface binding and annexin V+ EMP were more sharply increased in apoptosis than in activation. In contrast, in activation, inducible markers in EMP were markedly increased in both EMP and EC (CD62E>CD54>CD106). Coronary MaVEC released significantly less EMP than MiVEC.

CONCLUSION

EC release qualitatively and quantitatively distinct EMP during activation compared to apoptosis. Analysis of EMP phenotypic signatures may provide clinically useful information on the status of the endothelium.

摘要

背景

内皮细胞（EC）在激活和凋亡过程中会释放内皮微粒（EMP）。

目的

我们比较了三种细胞系在激活过程中与凋亡过程中释放的EMP种类的抗原表达情况。

方法

将来自肾和脑微血管（MiVEC）以及冠状动脉大血管（MaVEC）的EC与肿瘤坏死因子-α（TNF-α）共同孵育以诱导激活，或去除生长因子以诱导凋亡。通过流式细胞术检测EMP和EC上表达的抗原，包括组成性标志物（CD31、CD51/61、CD105）、诱导性标志物（CD54、CD62E和CD106）以及膜联蛋白V结合情况。

结果

发现在凋亡过程中，EMP中的组成性标志物显著增加（CD31>CD105），同时EC中的表达相应减少。膜联蛋白V在EC表面的结合以及膜联蛋白V阳性的EMP在凋亡过程中比在激活过程中增加得更为明显。相比之下，在激活过程中，EMP和EC中的诱导性标志物均显著增加（CD62E>CD54>CD106）。冠状动脉MaVEC释放的EMP明显少于MiVEC。