Pratelli Annamaria, Martella Vito, Decaro Nicola, Tinelli Antonella, Camero Michele, Cirone Francesco, Elia Gabriella, Cavalli Alessandra, Corrente Marialaura, Greco Grazia, Buonavoglia Domenico, Gentile Mattia, Tempesta Maria, Buonavoglia Canio
Department of Animal Health and Well-being, Faculty of Veterinary Medicine of Bari, S.p. per Casamassima km 3, 70010, Valenzano, Bari, Italy.
J Virol Methods. 2003 Jun 9;110(1):9-17. doi: 10.1016/s0166-0934(03)00081-8.
The sequence of the S gene of a field canine coronavirus (CCoV), strain Elmo/02, revealed low nucleotide (61%) and amino acid (54%) identity to reference CCoV strains. The highest correlation (77% nt and 81.7% aa) was found with feline coronavirus type I. A PCR assay for the S gene of strain Elmo/02 detected analogous CCoVs of different geographic origin, all which exhibited at least 92-96% nucleotide identity to each other and to strain Elmo/02. The evident genetic divergence between the reference CCoV strains and the newly identified Elmo/02-like CCoVs strongly suggests that a novel genotype of CCoV is widespread in the dog population.
一株犬冠状病毒(CCoV)田间毒株Elmo/02的S基因序列显示,与参考CCoV毒株的核苷酸同一性较低(61%),氨基酸同一性也较低(54%)。与I型猫冠状病毒的相关性最高(核苷酸77%,氨基酸81.7%)。针对Elmo/02毒株S基因的PCR检测发现了不同地理来源的类似CCoV,所有这些毒株相互之间以及与Elmo/02毒株的核苷酸同一性至少为92%-96%。参考CCoV毒株与新鉴定的类似Elmo/02的CCoV之间明显的遗传差异强烈表明,一种新型CCoV基因型在犬类群体中广泛存在。