脱氧肌苷激活的核苷酸酶在不同大鼠组织中的亚细胞分布及活性
Subcellular distribution and activity in different rat tissues of a deoxyinosine-activated nucleotidase.
作者信息
Tjernshaugen H, Fritzson P
出版信息
Biochem J. 1976 Jan 15;154(1):77-80. doi: 10.1042/bj1540077.
A nucleotidase (EC 3.1.3.31) isolated previously from rat liver cytosol was specifically measured in 14 different rat tissues and in subcellular fractions of liver and spleen, taking advantage of the stimulation exerted on it by deoxyinosine. The intracellular distribution studies showed that the enzyme is located almost entirely in the soluble cytoplasm except for the possible presence of 1-2% of the enzyme in the nucleus. The enzyme was present in various amounts in all the tissues studied. Spleen, thymus, and intestinal mucosa showed higher specific activities than any other tissue. On a per cell basis spleen, liver and intestinal mucosa had the highest enzyme activity, whereas bone marrow, brain, thymus, heart and skeletal muscle had activities in the lower range. The results may suggest that the enzyme plays a role in the recovery of endogenous nuclear material for nucleic acid synthesis.
利用脱氧肌苷对其产生的刺激作用，对先前从大鼠肝脏胞质溶胶中分离出的一种核苷酸酶（EC 3.1.3.31）在14种不同的大鼠组织以及肝脏和脾脏的亚细胞组分中进行了特异性测定。细胞内分布研究表明，该酶几乎完全位于可溶性细胞质中，细胞核中可能存在1-2%的该酶。在所研究的所有组织中，该酶的含量各不相同。脾脏、胸腺和肠黏膜的比活性高于其他任何组织。以每个细胞计算，脾脏、肝脏和肠黏膜的酶活性最高，而骨髓、脑、胸腺、心脏和骨骼肌的活性处于较低水平。这些结果可能表明该酶在回收用于核酸合成的内源性核物质中发挥作用。
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