Li J, Li Q, Li R
Department of Oncology, First Affiliated Hospital of Anhui Medical University, Hefei 230022, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 1999 Dec;13(4):377-9.
To investigate the presence of Epstein-Barr virus(EBV) gene in neck lymphatic metastasis of nasopharyngeal carcinoma.
Fifty patients' masses in the neck were punctured and the fluids were analyzed with PCR for EBV DNA.
Twenty seven cases showed EBV DNA positive among thirty cases of nasopharyngeal carcinoma with neck lymphatic metastasis, and the positive rate was 90%. One case was EBV DNA positive in seven cases of malignant lymphoma and the positive rate was 14.3%. One case was EBV DNA positive in eight tumor patients without NPC metastasis and the positive rate was 12.5%. Five cases with nasopharyngeal inflammation and neck lymphatic node proliferation were demonstrated to be EBV DNA negative.
This technique is rapid, sensitive and specific, which is valuable clinically, especially for the diagnosis in patients with cryptic nasopharyngeal carcinoma and with neck metastasis but lack of clear original focus.
探讨爱泼斯坦-巴尔病毒（EBV）基因在鼻咽癌颈部淋巴结转移灶中的存在情况。
对50例患者颈部肿物进行穿刺，抽取液体用聚合酶链反应（PCR）检测EBV DNA。
30例鼻咽癌颈部淋巴结转移患者中，27例EBV DNA阳性，阳性率为90%。7例恶性淋巴瘤患者中1例EBV DNA阳性，阳性率为14.3%。8例无鼻咽癌转移的肿瘤患者中1例EBV DNA阳性，阳性率为12.5%。5例鼻咽部炎症伴颈部淋巴结增生患者EBV DNA为阴性。
该技术快速、灵敏、特异，具有临床应用价值，尤其对隐匿性鼻咽癌伴颈部转移而原发灶不明确的患者诊断有重要意义。
