Dobrovol'skaia O B, Pershina L A, Kravtsova L A, Shchapova A I
Institute of Cytology and Genetics, Russian Academy of Sciences, Novosibirsk, 630090 Russia.
Genetika. 2003 Apr;39(4):570-4.
The effects of rye chromosomes 1R and 5R on androgenesis in cultured anthers of wheat--rye substitution lines was studied as dependent on the cultivar origin of the rye chromosomes and on the wheat genome (A or D) subjected to substitution. Chromosome 1R stimulated embryogenesis in anther cultures, while chromosome 5R suppressed it regardless of whether the corresponding wheat chromosomes were substituted in the A or D genome. The effect of chromosome 1R on embryogenesis proved to depend on its cultivar origin. Along with rye chromosome 1R, wheat chromosome 1A was shown to substantially affect total seedling regeneration. Regeneration of green seedlings was dramatically affected both by rye chromosome 1R and by wheat chromosome 1D. The results supported the published data that individual androgenesis parameters (embryogenesis, total plant regeneration, green plant regeneration) are controlled by different genetic mechanisms.
研究了黑麦1R和5R染色体对小麦-黑麦代换系花药培养中雄核发育的影响,该影响取决于黑麦染色体的品种来源以及被代换的小麦基因组(A或D)。1R染色体促进花药培养中的胚胎发生,而5R染色体则抑制胚胎发生,无论相应的小麦染色体是在A基因组还是D基因组中被代换。事实证明,1R染色体对胚胎发生的影响取决于其品种来源。与黑麦1R染色体一样,小麦1A染色体也被证明对总幼苗再生有显著影响。绿苗再生受到黑麦1R染色体和小麦1D染色体的显著影响。这些结果支持了已发表的数据,即个体雄核发育参数(胚胎发生、总植株再生、绿苗再生)受不同遗传机制控制。