Hsu Mayla, Zhang Jie, Flint Mike, Logvinoff Carine, Cheng-Mayer Cecilia, Rice Charles M, McKeating Jane A
Aaron Diamond AIDS Research Center and Center for the Study of Hepatitis C, Laboratory of Virology and Infectious Disease, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.
Proc Natl Acad Sci U S A. 2003 Jun 10;100(12):7271-6. doi: 10.1073/pnas.0832180100. Epub 2003 May 21.
HIV pseudotypes bearing native hepatitis C virus (HCV) glycoproteins (strain H and Con1) are infectious for the human hepatoma cell lines Huh-7 and PLC/PR5. Infectivity depends on coexpression of both E1 and E2 glycoproteins, is pH-dependent, and can be neutralized by mAbs mapping to amino acids 412-447 within E2. Cell-surface expression of one or all of the candidate receptor molecules (CD81, low-density lipoprotein receptor, scavenger receptor class B type 1, and dendritic cell-specific intercellular adhesion molecule 3 grabbing nonintegrin) failed to confer permissivity to HIV-HCV pseudotype infection. However, HIV-HCV pseudotype infectivity was inhibited by a recombinant soluble form of CD81 and a mAb specific for CD81, suggesting that CD81 may be a component of a receptor complex.
携带天然丙型肝炎病毒（HCV）糖蛋白（H株和Con1株）的HIV假型对人肝癌细胞系Huh-7和PLC/PR5具有感染性。感染性取决于E1和E2糖蛋白的共表达，呈pH依赖性，并且可被定位到E2内412 - 447位氨基酸的单克隆抗体中和。一种或所有候选受体分子（CD81、低密度脂蛋白受体、B类I型清道夫受体和树突状细胞特异性细胞间粘附分子3结合非整合素）的细胞表面表达未能赋予对HIV-HCV假型感染的易感性。然而，HIV-HCV假型的感染性被重组可溶性形式的CD81和针对CD81的单克隆抗体所抑制，这表明CD81可能是受体复合物的一个组成部分。
