Cui P, Chen W, Luo J
Department of Otorhinolaryngology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
Zhonghua Er Bi Yan Hou Ke Za Zhi. 2001 Feb;36(1):22-4.
To investigate the feasibility of fabricating a new cartilage in predetermined shapes with tissue engineering methods.
Human nasoseptal chondrocytes were seeded onto a nonwoven mesh of polyglycolic acid(PGA) to form a cell-PGA construct. The construction was then configured in sheet and tube shapes, and implanted subcutaneously into the dorsa of 11 athymic mice. The specimens were harvested 4, 6, 8 weeks after implantation and subjected to gross morphologic and histologic analysis.
Gross observation showed that the predetermined sheet and tube shapes of new cartilage were formed. Histological observation demonstrated that new mature cartilages were formed in 6-week. A Masson's trichrome stain showed the interwining bands of collagen at the periphery of the cartilage. Staining of Safranin O evaluated that the new cartilage was bound of glycosaminoglycan. In the control group, the PGA of the specimens were completely absorbed at 6 weeks.
Human nasoseptal chondrocytes-PGA construct could develop into a new cartilage in predetermined shapes in athymic mice.
探讨采用组织工程方法制造预定形状新软骨的可行性。
将人鼻中隔软骨细胞接种到聚乙醇酸（PGA）非织造网片上，形成细胞-PGA构建体。然后将构建体制作成片状和管状，皮下植入11只无胸腺小鼠的背部。在植入后4、6、8周收获标本，进行大体形态学和组织学分析。
大体观察显示形成了预定形状的片状和管状新软骨。组织学观察表明在6周时形成了新的成熟软骨。Masson三色染色显示软骨周边有相互交织的胶原带。番红O染色评估新软骨含有糖胺聚糖。在对照组中，标本的PGA在6周时完全吸收。
人鼻中隔软骨细胞-PGA构建体在无胸腺小鼠体内可发育成预定形状的新软骨。
