[豚鼠内淋巴囊钠钾ATP酶α和β亚基同工型的表达]
[Expression of alpha and beta subunit isoforms of Na, K-ATPase in the guinea pig endolymphatic sac].
作者信息
Zhong S, Mou Z, Liu Z, Chen J
机构信息
Department of Otorhinolaryngology Head and Neck Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China.
出版信息
Zhonghua Er Bi Yan Hou Ke Za Zhi. 2001 Apr;36(2):109-11.
OBJECTIVE
To investigate the expression and significance of alpha and beta subunit isoforms of Na, K-ATPase in the guinea pig endolymphatic sac.
METHODS
The distribution of alpha and beta subunit isoforms of Na, K-ATPase in endolymphatic sac of guinea pig was identified by immunocytochemistry and in situ hybridization.
RESULTS
The expression of Na, K-ATPase alpha and beta subunit isoforms varied among different cell regions of the endolymphatic sac. Epithelial cells of the endolymphatic sac were observed to contain the alpha 1, beta 1 and beta 2 subunit isoforms, and the expression of beta 2 was stronger than that of beta 1. Subepithelial cells contained alpha 1 and alpha 2 subunit isoforms and the expression of alpha 1 was stronger than that of alpha 2. No expression of alpha 3 subunit isoform was observed in endolymphatic sac.
CONCLUSION
Na, K-ATPase of endolymphatic sac consists of different alpha and beta subunit isoforms which, working in concert, serve to maintain homeostasis in inner ear.
目的
研究豚鼠内淋巴囊钠钾ATP酶α和β亚基异构体的表达及意义。
方法
采用免疫细胞化学和原位杂交技术，鉴定豚鼠内淋巴囊钠钾ATP酶α和β亚基异构体的分布。
结果
内淋巴囊不同细胞区域钠钾ATP酶α和β亚基异构体的表达各异。观察到内淋巴囊上皮细胞含有α1、β1和β2亚基异构体，且β2的表达强于β1。上皮下细胞含有α1和α2亚基异构体，且α1的表达强于α2。在内淋巴囊中未观察到α3亚基异构体的表达。
结论
内淋巴囊的钠钾ATP酶由不同的α和β亚基异构体组成，它们协同作用以维持内耳的稳态。
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