Department of Otolaryngology, Faculty of Medicine, Kagawa University, Kagawa 761-0793, Japan.
Neurosci Lett. 2012 Mar 28;513(1):47-50. doi: 10.1016/j.neulet.2012.02.005. Epub 2012 Feb 10.
A homeostasis of the electrochemical properties and volume of the endolymph in the inner ear is essential for hearing and equilibrium sensing and is maintained by ion-transport across an epithelial tissue, the endolymphatic sac. One of the key proteins in the maintenance is Na(+), K(+)-ATPase. Although we previously found that the Na(+), K(+)-ATPase in the sac plays a pivotal role in the control of the endolymphatic volume, the mechanism remains unclear. Therefore, in this study, we examined the expression of FXYD6, a functional modulator of the Na(+), K(+)-ATPase, in the epithelial cells of the endolymphatic sac using various approaches. Laser capture microdissection RT-PCR was used to identify FXYD6 mRNA in the endolymphatic sac. Immunolabeling with the specific antibody showed that FXYD6 was predominantly expressed in the intermediate portion of the endolymphatic sac, and it was colocalized with the Na(+), K(+)-ATPase. Because the Na(+), K(+)-ATPase in this region is known to exhibit a high level of activity, an interaction of FXYD6 with this transporter may be critically involved in the regulation of the characteristics of the endolymph.
内耳中内淋巴的电化学性质和体积的平衡对于听觉和平衡感至关重要，这种平衡是通过离子在耳内淋巴管上皮组织中的跨膜转运来维持的。维持平衡的关键蛋白质之一是 Na(+)，K(+) - ATP 酶。尽管我们之前发现囊中的 Na(+)，K(+) - ATP 酶在控制内淋巴体积方面起着关键作用，但机制尚不清楚。因此，在这项研究中，我们使用各种方法检查了 FXYD6（Na(+)，K(+) - ATP 酶的功能调节剂）在耳内淋巴管上皮细胞中的表达。使用激光捕获显微切割 RT-PCR 鉴定了内淋巴囊中 FXYD6 的 mRNA。用特异性抗体进行免疫标记显示，FXYD6 主要在内淋巴囊的中间部分表达，与 Na(+)，K(+) - ATP 酶共定位。因为该区域的 Na(+)，K(+) - ATP 酶已知具有高水平的活性，所以 FXYD6 与该转运蛋白的相互作用可能对调节内淋巴的特征起着至关重要的作用。
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