Azzar G, Rougier M, Berthillier G, Got R
Biochimie. 1976;58(3):285-95. doi: 10.1016/s0300-9084(76)80435-x.
Cat liver homogenates have been fractionated by differential centrifugation. Four particulate fractions (1 000 X g, 10 000 X g, and 145 000 X g) and a supernatant have been obtained. The biochemical composition of these fractions has been established from the assay and distribution pattern of 22 enzymatic and chemical constituents including marker enzymes for mitochondria, lysosomes, peroxisomes, plasma membranes, endoplasmic reticulum, Golgi apparatus and cell sap. The microsomal fraction was characterized by a moderate contamination with large cytoplasmic granules and by a low yield in protein and cholesterol. It contained 50 per cent of Golgi complex and about 40 per cent of plasma membranes. Morphological analysis of subcellular fractions was performed and confirmed biochemical results.
通过差速离心法对猫肝脏匀浆进行了分级分离。获得了四个颗粒级分（1000×g、10000×g和145000×g）以及一个上清液。这些级分的生化组成已根据22种酶和化学成分的测定及分布模式确定，这些成分包括线粒体、溶酶体、过氧化物酶体、质膜、内质网、高尔基体和细胞液的标记酶。微粒体级分的特征是受到大细胞质颗粒的中度污染，蛋白质和胆固醇产量低。它含有50%的高尔基体复合体和约40%的质膜。对亚细胞级分进行了形态学分析，并证实了生化结果。
