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[噬菌体T2L连接器的结构与蛋白质组成]

[Structure and protein composition of the bacteriophage T2L connector].

作者信息

Volkova T P, Pronina T A, Poglazov B F

出版信息

Biokhimiia. 1976 Jan;41(1):192-6.

PMID:1276258
Abstract

Methods of isolating structural bacteriophage T2 fragments containing: 1. a fragment consisting of a connector, tail tube and contracted sheath; 2. a fragment consisting of a free head, a connector and contracted sheath; 3. a fraction of some free tail tube and some free connectors; 4. a fraction of some free tail, free connectors and free fibers. The following parameters of connector consisting from a neck and a sleeve, which in its turn consists of a cap and a leg, are determined by means of electrone microscopy: 1) the length and the diameter of a cap and a sleeve being 45 and 145 A respectively; 2) the length and the diameter of a sleeve leg being 45 and 85 A respectively; 3) the length and the diameter of a connector neck being 85 and 70 A respectively. Polyacrylamide gel electrophoresis revealed in connectors proteins having molecular weight of 14 000, 15 000, 26 000 and 35 000 daltons.

摘要

分离结构噬菌体T2片段的方法,这些片段包括:1. 一个由连接头、尾管和收缩鞘组成的片段;2. 一个由游离头部、连接头和收缩鞘组成的片段;3. 一部分游离尾管和一些游离连接头;4. 一部分游离尾部、游离连接头和游离纤维。通过电子显微镜确定由颈部和套筒组成的连接头的以下参数,套筒又由帽和腿组成:1)帽和套筒的长度和直径分别为45 Å和145 Å;2)套筒腿的长度和直径分别为45 Å和85 Å;3)连接头颈部的长度和直径分别为85 Å和70 Å。聚丙烯酰胺凝胶电泳显示连接头中存在分子量为14000、15000、26000和35000道尔顿的蛋白质。

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