Ayna Buket, Celenk Sema, Atakul Fatma, Sezgin Behiye, Ozekinci Tuncer
University of Dicle, Department of Pedodontics, Diyarbakir City, Turkey.
J Dent Child (Chic). 2003 Jan-Apr;70(1):15-8.
Concern about the survival of microorganisms in deep carious lesions may often lead to unnecessary exposure of the pulp during excavation. This study evaluated the relationship between microbiological and clinical results of deep carious lesions in primary molars.
Clinical evaluation was performed on 72 deep carious lesions considered to have pulp perforation after traditional excavation. The dentin color and consistency were assessed by means of standardized scales using the technique of Bjorndal et al before restoration. For microbiological examination, dentin samples were taken by a sterile bur and transferred to transport fluid, then plated on tryptic soy agar for growth of total colony forming units. Samples werethen transferred on Rogosa SL agar for growth of oral lactobacilli and on mitis salivarius agar for growth of mutans streptococci.
The proportion of mutans streptococci, lactobacilli, and total colony forming units increased when the dentin color and consistency increased. However, the proportion of lactobacilli was smaller than that of mutans streptococci in carious dentin samples.
Contrary to expectations, the findings showed that the number of bacteria in carious dentin of primary molars was not significantly excessive.
对深龋病变中微生物存活情况的担忧常常会导致在去龋过程中不必要地暴露牙髓。本研究评估了乳牙深龋病变的微生物学与临床结果之间的关系。
对72例经传统去龋后被认为有牙髓穿孔的深龋病变进行临床评估。在修复前，采用比约恩达尔等人的技术，通过标准化量表评估牙本质的颜色和质地。进行微生物学检查时，用无菌钻取牙本质样本并转移至运输液中，然后接种于胰蛋白胨大豆琼脂上以培养总菌落形成单位。样本随后转移至罗戈萨SL琼脂上培养口腔乳杆菌，转移至轻唾琼脂上培养变形链球菌。
当牙本质颜色和质地加深时，变形链球菌、乳杆菌和总菌落形成单位的比例增加。然而，在龋坏牙本质样本中，乳杆菌的比例小于变形链球菌的比例。
与预期相反，研究结果表明乳牙龋坏牙本质中的细菌数量并非显著过多。
