氢氧化钙保护在乳牙间接盖髓术中的临床及微生物学效果
Clinical and microbiological effect of calcium hydroxide protection in indirect pulp capping in primary teeth.
作者信息
Pinto Alice Souza, de Araújo Fernando Borba, Franzon Renata, Figueiredo Marcia Cançado, Henz Sandra, García-Godoy Franklin, Maltz Marisa
机构信息
Pediatric Dentistry Program, College of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
出版信息
Am J Dent. 2006 Dec;19(6):382-6.
PURPOSE
To evaluate clinically and microbiologically the effect of calcium hydroxide (CH) on carious dentin on primary teeth submitted to indirect pulp capping (IPC).
METHODS
Twenty 4-7 year-old subjects with 42 treated teeth participated in the study. The treatment consisted of incomplete excavation of the demineralized dentin, application of a CH or gutta-percha (GP) layer and sealing with a resin-based composite for 4-7 months. After cavity preparation and sealing, the dentin was evaluated clinically (color and consistency) and microbiologically. Dentin samples were cultured on blood agar under aerobic and anaerobic conditions, in Mitis Salivarius agar and Rogosa SL agar.
RESULTS
39 teeth (20 in the CH Group and 19 in the GP Group) were reopened 4-7 months post-treatment for clinical and microbiological evaluation. In all teeth, the initial demineralized dentin was wet and soft or leathery. In the CH Group, 10% of the lesions were yellow, 80% light brown and 10% dark brown and in the GP Group, 94.7% were light brown and 5.3% dark brown. After treatment, the dentin was dry, 90% (Group CH) and 84.2% (Group GP) were dark brown. The final consistency was either leathery or hard. Three samples in the CH Group and five in Group GP changed from soft to leathery; only one sample (GP) remained leathery. 85% in the CH Group and 68.4% in GP Group turned hard after treatment. All bacterial counts decreased significantly by the end of treatment. In the CH Group, the bacterial (Log10 CFU + 1) anaerobic growth decreased from 4.84 +/- 1.31 to 1.35 +/- 1.54, aerobic from 4.09 +/- 1.04 to 0.92 +/- 1.30 and lactobacilli from 3.24 +/- 1.22 to 0.36 +/- 0.89, respectively; the mutans streptococci from 2.05 +/- 1.84 to 0.14 +/- 0.60. In GP Group, anaerobic growth decreased from 5.22 +/- 0.96 to 2.02 +/- 1.65 and aerobic from 4.23 +/- 1.37 to 1.08 +/- 1.29 and lactobacilli from 2.06 +/- 1.81 to 0.00 +/- 0.00, respectively; the mutans streptococci from 3.16 +/- 1.59 to 0.28 +/- 0.84.
目的
从临床和微生物学角度评估氢氧化钙（CH）对接受间接盖髓术（IPC）的乳牙龋坏牙本质的作用。
方法
20名4 - 7岁的受试者，其42颗牙齿接受了治疗，参与了本研究。治疗包括不完全去除脱矿牙本质，应用一层CH或牙胶（GP），并用树脂基复合材料密封4 - 7个月。在窝洞预备和密封后，对牙本质进行临床（颜色和质地）及微生物学评估。牙本质样本在血琼脂上进行需氧和厌氧培养，以及在轻唾琼脂和罗氏SL琼脂上培养。
结果
治疗后4 - 7个月，39颗牙齿（CH组20颗，GP组19颗）被重新打开进行临床和微生物学评估。在所有牙齿中，最初的脱矿牙本质湿润、柔软或呈皮革样。在CH组中，10%的病变为黄色，80%为浅棕色，10%为深棕色；在GP组中，94.7%为浅棕色，5.3%为深棕色。治疗后，牙本质干燥，CH组90%和GP组84.2%为深棕色。最终质地为皮革样或坚硬。CH组3个样本和GP组5个样本从柔软变为皮革样；只有1个样本（GP组）仍为皮革样。CH组85%和GP组中68.4%在治疗后变硬。到治疗结束时，所有细菌计数均显著下降。在CH组中，细菌（Log10 CFU + 1）厌氧生长从4.84 ± 1.31降至1.35 ± 1.54，需氧生长从4.09 ± 1.04降至0.92 ± 1.30，乳酸杆菌从3.24 ± 1.22降至0.36 ± 0.89；变形链球菌从2.05 ± 1.84降至0.14 ± 0.60。在GP组中，厌氧生长从5.22 ± 0.96降至2.02 ± 1.65，需氧生长从4.23 ± 1.37降至1.08 ± 1.29，乳酸杆菌从2.06 ± 1.81降至0.00 ± 0.00；变形链球菌从3.16 ± 1.59降至0.28 ± 0.84。
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