Guittat Lionel, Alberti Patrizia, Rosu Frédéric, Van Miert Sabine, Thetiot Emilie, Pieters Luc, Gabelica Valérie, De Pauw Edwin, Ottaviani Alexandre, Riou Jean-François, Mergny Jean-Louis
Laboratoire de Biophysique, Muséum National d'Histoire Naturelle, INSERM UR 565, CNRS UMR 8646, 43, rue Cuvier, 75231 Paris cedex 5, France.
Biochimie. 2003 May;85(5):535-47. doi: 10.1016/s0300-9084(03)00035-x.
Cryptolepine, the main alkaloid present in the roots of Cryptolepis sanguinolenta, presents a large spectrum of biological properties. It has been reported to behave like a DNA intercalator with a preference for GC-rich sequences. In this study, dialysis competition assay and mass spectrometry experiments were used to determine the affinity of cryptolepine and neocryptolepine for DNA structures among duplexes, triplexes, quadruplexes and single strands. Our data confirm that cryptolepine and neocryptolepine prefer GC over AT-rich duplex sequences, but also recognize triplex and quadruplex structures. These compounds are weak telomerase inhibitors and exhibit a significant preference for triplexes over quadruplexes or duplexes.
隐丹参酮是红背菜根中的主要生物碱，具有广泛的生物学特性。据报道，它表现得像一种DNA嵌入剂，偏好富含GC的序列。在本研究中，采用透析竞争测定法和质谱实验来确定隐丹参酮和新隐丹参酮对双链体、三链体、四链体和单链等DNA结构的亲和力。我们的数据证实，隐丹参酮和新隐丹参酮相较于富含AT的双链序列更偏好GC序列，但也能识别三链体和四链体结构。这些化合物是弱端粒酶抑制剂，并且相较于四链体或双链体，它们对三链体表现出显著的偏好。
