Hotta Yuko, Honda Takao, Naito Makoto, Kuwano Ryozo
Department of Molecular Genetics, Genome Science Branch, Center for Bioresource-based Researches, Brain Research Institute, Niigata University, Niigata, Japan.
Brain Res Dev Brain Res. 2003 Jun 12;143(1):1-13. doi: 10.1016/s0165-3806(03)00035-x.
Mouse coxsackie virus and adenovirus receptor (mCAR), which was isolated from the nerve growth cone-enriched fraction of newborn mouse brains, is a member of immunoglobulin-super family, and functions as a homophilic adhesion molecule. We observed the expression of mCAR in embryos to adult tissues by means of immunohistochemical analysis with a peptide antibody. mCAR expression was first detected in the embryonic ectoderm in the uterus on embryonic day 6.5 (E6.5). Then it was strongly expressed in the neuroepithelium of the neural tube, the developing brain and the spinal cord from E8.5 to postnatal day 7 (P7), in the cranial motor nerves from E9.5 to E11.5, and in the optic nerve from E13.5 to P7, which agrees with periods of their respective morphogenetic peaks. This expression of mCAR decreased postnatally and was absent in adult tissues. We found that mCAR occurred in a few proliferating cells of the hippocampal dentate gyrus, the subventricular zone (SVZ) of the lateral ventricles, and the rostral migratory stream (RMS) over P21. These observations demonstrate that mCAR was expressed characteristically in the immature neuroepithelium including progenitor cells or radial cells derived from the neural tube and in immature cells in a selected germinal zone of the mature brain. Based on our findings, we propose that mCAR is involved in migration and fasciculation during a restricted period as an adhesion molecule.
从小鼠新生脑富含神经生长锥的部分分离出的小鼠柯萨奇病毒和腺病毒受体（mCAR）是免疫球蛋白超家族的成员，作为一种同源性黏附分子发挥作用。我们用肽抗体通过免疫组织化学分析观察了mCAR在胚胎到成年组织中的表达情况。mCAR的表达最早在胚胎第6.5天（E6.5）子宫内的胚胎外胚层中被检测到。然后在胚胎第8.5天到出生后第7天（P7），它在神经管的神经上皮、发育中的脑和脊髓中强烈表达，在胚胎第9.5天到第11.5天在颅运动神经中表达，在胚胎第13.5天到P7在视神经中表达，这与它们各自形态发生高峰的时期一致。mCAR的这种表达在出生后下降，在成年组织中不存在。我们发现，在出生后第21天之后，mCAR出现在海马齿状回、侧脑室室下区（SVZ）和吻侧迁移流（RMS）的一些增殖细胞中。这些观察结果表明，mCAR在包括源自神经管的祖细胞或放射状细胞的未成熟神经上皮以及成熟脑的选定生发区的未成熟细胞中具有特征性表达。基于我们的发现，我们提出mCAR在一个受限时期作为黏附分子参与迁移和束状化过程。
