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[大鼠心肌的酶异质性]

[Enzymatic heterogeneity of the rat myocardium].

作者信息

Klibaner M I, Danilova K M

出版信息

Biull Eksp Biol Med. 1976;81(4):487-9.

PMID:1276459
Abstract

The histoenzymatic method was applied to the study of distribution of the activity of the redox enzymes in the myocardium of the ventricles in rats; distribution of the activity of lactic and malic dehydrogenase and of alpha-glycerophosphate proved to be the most manifest near the apex of the heart and was expressed in the presence of "spotty" areas of increased activity against the general homogeneous background of formazan deposits. The activity of mitochondrial upsilon-glycerophoric dehydrogenase was seen in all the portions of the ventricles and was characterized by an uneven distribution in the sarcoplasm with increase in the direction from the interdisc to the nucleus. Unevenness of distribution of the beta-oxybutyric dehydrogenase activity was detected in some of the animals and was pronounced in all the portions of the myocardium. The intensity of the reaction in detection of succinic dehydrogenase, NAD- and NADP-diaphorases varied but insignificantly.

摘要

采用组织酶法研究大鼠心室心肌中氧化还原酶活性的分布;乳酸脱氢酶、苹果酸脱氢酶和α-甘油磷酸脱氢酶的活性分布在心脏尖端附近最为明显,表现为在azan沉淀的一般均匀背景下存在“斑点状”活性增加区域。线粒体γ-甘油磷酸脱氢酶的活性在心室的所有部分均可见,其特征是在肌浆中分布不均匀,从闰盘向细胞核方向增加。在一些动物中检测到β-羟丁酸脱氢酶活性分布不均匀,且在心肌的所有部分都很明显。琥珀酸脱氢酶、NAD-和NADP-黄递酶检测中的反应强度有所变化,但不显著。

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