Humiczewska M
Folia Histochem Cytochem (Krakow). 1975;13(3-4):161-74.
The object of the study was to investigate the occurrence and localization of oxidative enzymes in the redia -- the third larval stage of Fasciola hepatica L. The author detected cytochrome oxidase, peroxidase, NADH and NADPH tetrazolium reductases (diaphorases), as well as succinate, isocitrate, malate, lactate, alpha-glycerophosphate, glyceraldehyde phosphate, glucose-6-phosphate, 6-phosphogluconate, beta-hydroxybutyrate, L-glutamate, and alcohol dehydrogenases. The presence and localization of the enzymes in various periods of development of the redia were detected with histochemical methods. Out of the studied oxidases and dehydrogenases only cytochrome oxidase was found to be absent from the stages of young rediae. It was ascertained that the redia uses all three paths of release of energy i.e. the glycolytic, Krebs, and pentose cycles, glycolysis being presumably the principal mode of energy production.
本研究的目的是调查肝片吸虫第三幼虫期雷蚴体内氧化酶的发生情况及定位。作者检测到了细胞色素氧化酶、过氧化物酶、NADH和NADPH四氮唑还原酶（黄递酶），以及琥珀酸脱氢酶、异柠檬酸脱氢酶、苹果酸脱氢酶、乳酸脱氢酶、α-甘油磷酸脱氢酶、磷酸甘油醛脱氢酶、葡萄糖-6-磷酸脱氢酶、6-磷酸葡萄糖酸脱氢酶、β-羟丁酸脱氢酶、L-谷氨酸脱氢酶和乙醇脱氢酶。采用组织化学方法检测了雷蚴不同发育时期这些酶的存在情况及定位。在所研究的氧化酶和脱氢酶中，仅发现幼龄雷蚴阶段不存在细胞色素氧化酶。已确定雷蚴利用能量释放的所有三条途径，即糖酵解、三羧酸循环和戊糖循环，其中糖酵解可能是主要的能量产生方式。
