Clynen Elke, De Loof Arnold, Schoofs Liliane
Laboratory for Developmental Physiology and Molecular Biology, Zoological Institute, K.U. Leuven, Naamsestraat 59, BE-3000 Leuven, Belgium.
Gen Comp Endocrinol. 2003 Jun 1;132(1):1-9. doi: 10.1016/s0016-6480(03)00158-8.
In 2002, the Nobel Prize for chemistry was awarded to the inventors of two novel ionization techniques in mass spectrometry, MALDI and ESI. These techniques, often in combination with data from genomic databases, represent an extremely powerful tool in analytical (bio)chemistry, with many applications, e.g., in the field of proteomics. Peptides, which are small proteins, have, despite their importance as controlling agents in numerous physiological processes, as yet been much less intensively studied by these novel techniques than larger proteins. The term peptidomics, i.e., the study of all peptides expressed by a certain cell, organ or organism was only introduced in 2001. In neuroendocrinology, spectacular progress could already be realized and the future looks bright. In this minireview we discuss the different methodologies that are used in peptidomics and give an overview of the wide range of applications.
2002年，诺贝尔化学奖授予了质谱分析中两种新型电离技术——基质辅助激光解吸电离（MALDI）和电喷雾电离（ESI）的发明者。这些技术常常与基因组数据库的数据相结合，是分析（生物）化学中极为强大的工具，有许多应用，例如在蛋白质组学领域。肽作为小蛋白质，尽管在众多生理过程中作为调控因子很重要，但与较大蛋白质相比，这些新技术对其研究的深入程度仍要低得多。“肽组学”一词，即对特定细胞、器官或生物体表达的所有肽进行研究，直到2001年才被提出。在神经内分泌学领域，已经取得了显著进展，前景光明。在这篇微型综述中，我们讨论了肽组学中使用的不同方法，并概述了其广泛的应用。
