子痫前期妊娠胎盘基底板中胰岛素样生长因子-II及胰岛素样生长因子结合蛋白-1的表达

Expression of insulin-like growth factor-II and insulin-like growth factor binding protein-1 in the placental basal plate from pre-eclamptic pregnancies.

作者信息

Shin J C, Lee J H, Yang D E, Moon H B, Rha J G, Kim S P

机构信息

Department of Obstetrics and Gynecology, Division of Maternal-Fetal Medicine, Medical College, Catholic University of Korea, Seoul, South Korea.

出版信息

Int J Gynaecol Obstet. 2003 Jun;81(3):273-80. doi: 10.1016/s0020-7292(02)00444-7.

DOI:10.1016/s0020-7292(02)00444-7

PMID:12767569

Abstract

OBJECTIVES

To determine whether expressions of insulin-like growth factor-II (IGF-II) and insulin-like growth factor binding protein-1 (IGFBP-1) are altered in pre-eclamptic placenta and to elucidate the possible relationship between their expressions and a mechanism for inadequate trophoblast invasion in pre-eclampsia.

METHODS

Placental tissues were obtained at cesarean delivery from five normotensive, nine mild pre-eclamptic and five severe pre-eclamptic women at 33-39 completed weeks of gestation. After total ribonucleic acid was extracted, reverse transcriptase-polymerase chain reaction was performed to determine IGF-II and IGFBP-1 mRNA expression. Product bands were quantitated by scanning densitometry and results were expressed as ratio of cytokines/beta-actin. Western blot analysis was also done to determine IGF-II and IGFBP-1 protein expression. Statistical analysis was determined by Kruskal-Wallis analysis of variance with the Scheffe multiple post-hoc test.

RESULTS

The IGF-II mRNA levels of mild and severe pre-eclamptic placenta were significantly lower than that of uncomplicated placenta (P<0.005, P<0.001, respectively), with the level of severe pre-eclamptic placenta being significantly lower than that of mild pre-eclamptic placenta (P<0.05). As for the IGF-II protein expression, a significant decrease was found among the three groups (P<0.001), correlating with the IGF-II mRNA results. However, the mean IGFBP-1 mRNA levels of mild and severe pre-eclamptic placenta were significantly higher than that of uncomplicated placenta (P<0.05, P<0.005, respectively), with the level of severe pre-eclamptic placenta being significantly raised compared with that of mild pre-eclamptic placenta (P<0.05). Finally, a significant increase of IGFBP-1 protein expression was noted among the three groups (P<0.001), correlating with the IGFBP-1 mRNA results.

CONCLUSIONS

This study suggests that IGF-II and IGFBP-1 might be associated with the impaired trophoblastic invasion that may lead to pathogenesis of pre-eclampsia.

摘要

目的

确定胰岛素样生长因子-II（IGF-II）和胰岛素样生长因子结合蛋白-1（IGFBP-1）在子痫前期胎盘中的表达是否改变，并阐明它们的表达与子痫前期滋养层细胞侵袭不足机制之间的可能关系。

方法

在剖宫产时从妊娠33 - 39周的5名血压正常、9名轻度子痫前期和5名重度子痫前期孕妇获取胎盘组织。提取总核糖核酸后，进行逆转录聚合酶链反应以测定IGF-II和IGFBP-1 mRNA表达。通过扫描光密度法对产物条带进行定量，结果以细胞因子/β-肌动蛋白的比值表示。还进行了蛋白质印迹分析以测定IGF-II和IGFBP-1蛋白表达。采用Kruskal-Wallis方差分析及Scheffe多重事后检验进行统计分析。

结果

轻度和重度子痫前期胎盘的IGF-II mRNA水平显著低于正常胎盘（分别为P<0.005，P<0.001），重度子痫前期胎盘的水平显著低于轻度子痫前期胎盘（P<0.05）。至于IGF-II蛋白表达，三组间有显著下降（P<0.001），与IGF-II mRNA结果相关。然而，轻度和重度子痫前期胎盘的平均IGFBP-1 mRNA水平显著高于正常胎盘（分别为P<0.05，P<0.005），重度子痫前期胎盘的水平与轻度子痫前期胎盘相比显著升高（P<0.05）。最后，三组间IGFBP-1蛋白表达有显著增加（P<0.001），与IGFBP-1 mRNA结果相关。