诺如病毒坎伯韦尔蛋白酶的反式活性及ORF1编码的N端蛋白的切割
Trans activity of the norovirus Camberwell proteinase and cleavage of the N-terminal protein encoded by ORF1.
作者信息
Seah Ee Ling, Marshall John A, Wright Peter J
机构信息
Department of Microbiology, Monash University, Clayton, Victoria 3800, Australia.
出版信息
J Virol. 2003 Jun;77(12):7150-5. doi: 10.1128/jvi.77.12.7150-7155.2003.
Abstract
The virus-encoded proteinase of Camberwell virus, a genogroup 2 norovirus, was synthesized in Escherichia coli. The purified proteinase had correct N and C termini and showed trans activity in cell-free assays. trans activity was also demonstrated in COS cells transfected with constructs encoding either the proteinase or a proteinase-polymerase fusion. The N-terminal protein of ORF1 was cleaved in COS cells, possibly at the site E(194)/S.
摘要
坎伯韦尔病毒（一种基因组2型诺如病毒）的病毒编码蛋白酶在大肠杆菌中合成。纯化后的蛋白酶具有正确的N端和C端，并在无细胞试验中显示出反式活性。在用编码蛋白酶或蛋白酶 - 聚合酶融合蛋白的构建体转染的COS细胞中也证实了反式活性。ORF1的N端蛋白在COS细胞中被切割，可能在E(194)/S位点。
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1
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Virus Res. 2002 Oct;89(1):29-39. doi: 10.1016/s0168-1702(02)00114-4.
2
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Arch Virol. 2002 Aug;147(8):1655-63. doi: 10.1007/s007050200039.
3
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J Virol. 2002 Sep;76(17):8582-95. doi: 10.1128/jvi.76.17.8582-8595.2002.
4
Subdomain specific functions of the RNA polymerase region of poliovirus 3CD polypeptide.
Virology. 2002 Jul 5;298(2):200-13. doi: 10.1006/viro.2002.1481.
5
Processing map and essential cleavage sites of the nonstructural polyprotein encoded by ORF1 of the feline calicivirus genome.
J Virol. 2002 Jul;76(14):7060-72. doi: 10.1128/jvi.76.14.7060-7072.2002.
6
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J Virol. 2002 Jun;76(12):5949-58. doi: 10.1128/jvi.76.12.5949-5958.2002.
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J Med Virol. 2002 Mar;66(3):400-6. doi: 10.1002/jmv.2159.
8
Gastroenteritis viruses: an overview.
Novartis Found Symp. 2001;238:5-19; discussion 19-25. doi: 10.1002/0470846534.ch2.
9
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J Virol. 2001 Feb;75(3):1211-9. doi: 10.1128/JVI.75.3.1211-1219.2001.
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