[白细胞介素-5在人鼻息肉组织中的浓度及表达]

[The concentration and expression of IL-5 in human nasal polyp tissue].

作者信息

Xu R, Li Y, Xie M, Xu G, Zhang G, Wang S

机构信息

Department of Otolaryngology, Third Affiliated Hospital, Sun Yat-Sen University of Medical Sciences, Guangzhou 510630, China.

出版信息

Zhonghua Er Bi Yan Hou Ke Za Zhi. 2000 Aug;35(4):251-4.

PMID:12768697

Abstract

OBJECTIVE

To study the concentration and expression of IL-5 in nasal polyp tissues and explore its significance in the micro-environment differentiation of eosinophils accumulation and clarify the conception of nasal polyposis.

METHODS

The concentration and expression of IL-5 in nasal polyp tissues of 40 patients were determined by ELISA and immunohistochemistry, and inferior turbinate mucosa from patients with nasal polyps and healthy volunteers was used as control.

RESULTS

IL-5 concentration in the polyp tissues was significantly higher than that in inferion turbinate mucosa(P < 0.05). There was no significant difference in inferion turbinate mucosa between the patients with nasal polyps and healthy volunteers (P > 0.05). IL-5 concentration in polyp tissues was markedly higher in patients with extensive polypoid change of nasal mucosa, history of previous polypectomy and allergic rhinitis compared with those without these features (P < 0.05). IL-5 concentration had no correlation with age and sex (P > 0.05). 2. 80.1% of the eosinophils were positive for IL-5 and 90.9% of IL-5 positive cells were eosinophils. Only 3.7% of the lymphocytes and neutrophils were IL-5 positive, and IL-5 was not detectable in epithelial cells. IL-5 expression in eosinophils of polyp tissues was remarkably stronger than that of the turbinate mucosa (P < 0.05). There was no significant difference in inferion turbinate mucosa between the patients with nasal polyps and healthy volunteers (P > 0.05). IL-5 expression of eosinophils in polyp tissues was significantly stronger in patients with extensive polypoid change of nasal mucosa, history of previous polypectomy and allergic rhinitis compared with those without these features (P < 0.05). There was no significant difference in IL-5 expression in lymphocytes and neutrophils between polyp tissues and inferior turbinate nasal mucosa (both P > 0.05).

CONCLUSION

IL-5 is a key protein in eosinophilic pathologic mechanisms in nasal polyp tissues.

摘要

目的

研究白细胞介素-5（IL-5）在鼻息肉组织中的浓度及表达情况，探讨其在嗜酸性粒细胞聚集微环境分化中的意义，阐明鼻息肉病的概念。

方法

采用酶联免疫吸附测定（ELISA）法和免疫组织化学法检测40例患者鼻息肉组织中IL-5的浓度及表达情况，以鼻息肉患者的下鼻甲黏膜及健康志愿者的下鼻甲黏膜作为对照。

结果

鼻息肉组织中IL-5浓度显著高于下鼻甲黏膜（P<0.05）。鼻息肉患者与健康志愿者的下鼻甲黏膜中IL-5浓度差异无统计学意义（P>0.05）。与无广泛鼻黏膜息肉样改变、既往息肉切除史及变应性鼻炎的患者相比，有上述特征的患者鼻息肉组织中IL-5浓度明显更高（P<0.05）。IL-5浓度与年龄和性别无关（P>0.05）。2. 80.1%的嗜酸性粒细胞IL-5呈阳性，90.9%的IL-5阳性细胞为嗜酸性粒细胞。仅3.7%的淋巴细胞和中性粒细胞IL-5呈阳性，上皮细胞中未检测到IL-5。鼻息肉组织中嗜酸性粒细胞的IL-5表达明显强于下鼻甲黏膜（P<0.05）。鼻息肉患者与健康志愿者的下鼻甲黏膜中IL-5表达差异无统计学意义（P>0.05）。与无广泛鼻黏膜息肉样改变、既往息肉切除史及变应性鼻炎的患者相比，有上述特征的患者鼻息肉组织中嗜酸性粒细胞的IL-5表达明显更强（P<0.05）。鼻息肉组织与下鼻甲鼻黏膜中淋巴细胞和中性粒细胞的IL-5表达差异均无统计学意义（均为P>0.05）。