Jouanin L, Pham-Delegue M -H., Bonadé-Bottino M, Girard C
Laboratoire de Biologie Cellulaire, INRA de Versailles, Route de St Cyr, 78026, Versailles Cedex, France
J Insect Physiol. 1998 Jul;44(7-8):569-577. doi: 10.1016/s0022-1910(98)00050-x.
The aim of this study was to assess the potential effect of a transgenic line of oilseed rape expressing oryzacystatin I (OCI) on two strains of cabbage seed weevil. The level of OCI expression in seeds was approximately 0.05% of total soluble proteins. The insects were field-collected in two different locations, and their progeny was analyzed after a 3 week-development in pods. Both strains showed a similar pattern of proteolytic activity, and similar levels of OCI-sensitive proteinase activity in vitro. However, the larvae showed differential susceptibility to the transgenic plants. Despite inhibition of digestive proteinases in vitro by OCI in both strains, one strain showed an increased growth rate when fed the transgenic seeds, while the other strain remained unaffected. While suggesting the importance of studying individuals from different populations when assessing the effect of proteinase inhibitor-expressing plants on insect growth, our results also point out the necessity of studying the biochemical interactions taking place in vivo between the recombinant inhibitors and their target proteinases.
本研究的目的是评估表达水稻巯基蛋白酶抑制剂I（OCI）的转基因油菜品系对两种甘蓝籽象鼻虫菌株的潜在影响。种子中OCI的表达水平约占总可溶性蛋白的0.05%。这些昆虫是在两个不同地点野外采集的，其后代在豆荚中发育3周后进行分析。两种菌株都表现出相似的蛋白水解活性模式，以及体外相似水平的OCI敏感蛋白酶活性。然而，幼虫对转基因植物表现出不同的敏感性。尽管两种菌株的体外消化蛋白酶都被OCI抑制，但当喂食转基因种子时，一种菌株的生长速率增加，而另一种菌株则不受影响。我们的研究结果表明，在评估表达蛋白酶抑制剂的植物对昆虫生长的影响时，研究不同种群个体的重要性，同时也指出了研究重组抑制剂与其靶蛋白酶在体内发生的生化相互作用的必要性。
