Jouanin L, Pham-Delegue M -H., Bonadé-Bottino M, Williams I, Bartlet E, Zaccomer B, Le Métayer M, Girard C
Laboratoire de Biologie Cellulaire, INRA de Versailles, Route de St Cyr, 78026, Versailles, France
J Insect Physiol. 1998 Mar;44(3-4):263-270. doi: 10.1016/s0022-1910(97)00142-x.
The resistance of a transgenic line of oilseed rape expressing constitutively the cysteine proteinase inhibitor oryzacystatin I (OCI) was assessed against Psylliodes chrysocephala L. (Coleoptera: Chrysomelidae). The levels of OCI expression in the transformed line averaged 0.2% and 0.05% of total soluble protein in leaves and petioles respectively. In vitro analyses showed that P. chrysocephala larvae use both cysteine and serine proteinases for protein digestion, and that all the cysteine proteolytic activity is OCI-sensitive. However, bioassays showed that adults fed identically on leaf discs from control or transformed plants. When larvae were reared on transgenic plants expressing OCI, they showed an increase in weight gain compared to those reared on control plants. Furthermore, those larvae from transgenic plants exhibited a 2-fold increase in both cysteine and serine proteolytic activity as a reponse to the presence of OCI. The plasticity of insect digestive physiology and feeding behaviour are discussed, as well as the relevance of engineering a genotype expressing both types of proteinase inhibitors.
对组成型表达半胱氨酸蛋白酶抑制剂水稻胱抑素I（OCI）的转基因油菜品系进行了针对黄曲条跳甲（鞘翅目：叶甲科）的抗性评估。在转化品系中，OCI在叶片和叶柄中的表达水平分别平均占总可溶性蛋白的0.2%和0.05%。体外分析表明，黄曲条跳甲幼虫利用半胱氨酸蛋白酶和丝氨酸蛋白酶进行蛋白质消化，并且所有半胱氨酸蛋白酶活性都对OCI敏感。然而，生物测定表明，成虫取食对照或转基因植物的叶盘时情况相同。当幼虫在表达OCI的转基因植物上饲养时，与在对照植物上饲养的幼虫相比，它们的体重增加有所增加。此外，来自转基因植物的幼虫在存在OCI的情况下，半胱氨酸和丝氨酸蛋白酶活性均增加了两倍。文中讨论了昆虫消化生理和取食行为的可塑性，以及构建表达两种类型蛋白酶抑制剂的基因型的相关性。
