Zimmerman R J, Kloos W E
Can J Microbiol. 1976 Jun;22(6):771-9. doi: 10.1139/m76-113.
The electrophoretic mobilities of non-specific esterases in vertical polyacrylamide slab gels were determined for 184 strains of staphylococci, representing a total of 18 proposed species and subspecies. Markedly uniform esterase patterns were seen within species demonstrating a high degree of human host specificity, while those species demonstrating a wide host range were polytypic and often showed considerable polymorphism. The unique banding patterns found in several species indicate that this technique may serve as a valuable aid to existing taxonomic schemes. Starch gel electrophoresis of representative strains usually produced sharper esterase bands than were found with polyacrylamide electrophoresis. However, the additional molecular-sieving effect produced by the polyacrylamide gels differentiated esterases to a greater extent.
对184株葡萄球菌（代表总共18个拟议的种和亚种）进行了垂直聚丙烯酰胺平板凝胶中非特异性酯酶的电泳迁移率测定。在种内观察到明显一致的酯酶图谱，表明对人类宿主具有高度特异性，而那些具有广泛宿主范围的种则是多型的，并且常常表现出相当大的多态性。在几个种中发现的独特条带模式表明，这项技术可能有助于现有的分类方案。代表性菌株的淀粉凝胶电泳通常产生比聚丙烯酰胺电泳更清晰的酯酶条带。然而，聚丙烯酰胺凝胶产生的额外分子筛效应在更大程度上区分了酯酶。
