Goullet P, Picard B
J Gen Microbiol. 1984 Jun;130(6):1471-80. doi: 10.1099/00221287-130-6-1471.
Esterases of 53 strains of Yersinia enterocolitica sensu stricto, including five previously defined biotypes, and 30 strains of Yersinia pseudotuberculosis were analysed by horizontal polyacrylamide-agarose gel electrophoresis and by isoelectrofocusing in thin-layer polyacrylamide gel. Esterase bands were defined by their range of activity towards several synthetic substrates, their resistance to heat and to di-isopropyl fluorophosphate. The two species were characterized by distinct electrophoretic patterns of their esterases. The apparent molecular weights of the heat-resistant esterase of Y. enterocolitica and of the major heat-resistant esterase of Y. pseudotuberculosis, as determined by polyacrylamide gradient gel electrophoresis, were estimated to be 52 000 and 250 000, respectively. On the basis of electrophoretic mobilities and isoelectric points of esterases produced by strains of Y. enterocolitica, five principal zymotypes were observed: two for strains of biotype 1, two for strains of biotypes 2 and 3, respectively, and only one for strains of both biotypes 4 and 5. The zymotypes of strains of biotypes 2, 3, 4 and 5 appeared to be more closely related to one another than to zymotypes of strains of biotype 1. Variations in number or mobility of bands observed within each biotype of Y. enterocolitica and within some serotypes of Y. pseudotuberculosis could represent an additional marker for epidemiological analysis.
采用水平聚丙烯酰胺 - 琼脂糖凝胶电泳以及在薄层聚丙烯酰胺凝胶中进行等电聚焦的方法，对53株小肠结肠炎耶尔森菌（包括先前定义的5个生物型）和30株假结核耶尔森菌的酯酶进行了分析。酯酶条带是根据它们对几种合成底物的活性范围、对热和二异丙基氟磷酸的抗性来定义的。这两个菌种的酯酶具有明显不同的电泳图谱。通过聚丙烯酰胺梯度凝胶电泳测定，小肠结肠炎耶尔森菌耐热酯酶和假结核耶尔森菌主要耐热酯酶的表观分子量分别估计为52000和250000。根据小肠结肠炎耶尔森菌菌株产生的酯酶的电泳迁移率和等电点，观察到了5种主要的酶型：生物型1的菌株有2种，生物型2和3的菌株分别有2种，生物型4和5的菌株都只有1种。生物型2、3、4和5的菌株的酶型彼此之间似乎比与生物型1的菌株的酶型关系更密切。在小肠结肠炎耶尔森菌的每个生物型以及假结核耶尔森菌的一些血清型中观察到的条带数量或迁移率的变化可能代表了用于流行病学分析的额外标记。
