Rouleux-Bonnin F, Renault S, Rabouille A, Periquet G, Bigot Y
Institut de Recherche sur la Biologie de l'Insecte, Faculté des Sciences, UPRESA 6035, Parc de Granmont, F-37200, Tours, France
J Insect Physiol. 1999 May;45(5):479-484. doi: 10.1016/s0022-1910(98)00149-8.
In braconid species, teratocytes are derived from a serosal cell membrane which envelops the developing parasitoid embryo. On hatching, this membrane dissociates into individual cells, the teratocytes, which then circulate in the haemolymph of the host. We describe herein such a membrane, surrounding the embryo in eggs of the ichneumonid parasitoid wasp, Diadromus pulchellus. This membrane consisted of a single sheet of tightly packed cells with large 12+/-1.4 &mgr;m nuclei. These cells were released after hatching in vitro and cells of the same size were detected in vivo, in the vicinity of the D. pulchellus embryo. The number of nuclei detected suggests that the serosal membrane consists of about 450+/-150 cells. These cells did not grow after hatching of the parasitoid egg in the parasitized host, Acrolepiosis assectella, during the development of the parasitoid wasp larva. Southern blot experiments, using D. pulchellus satellite DNA or the ribosomal genes as probes, showed that free-living floating cells of wasp origin were present in the body of the parasitized host. This is the first time that free-floating teratocyte-like cells have been described in species of the Ichneumonidae.
在茧蜂种类中，畸形细胞源自包裹发育中寄生蜂胚胎的浆膜细胞膜。孵化时，该膜解离成单个细胞，即畸形细胞，然后在宿主的血淋巴中循环。我们在此描述了一种膜，它围绕着姬蜂科寄生蜂——美丽潜蛾姬蜂（Diadromus pulchellus）卵中的胚胎。该膜由单层紧密排列的细胞组成，细胞核大，直径为12±1.4微米。这些细胞在体外孵化后释放出来，并且在体内，在美丽潜蛾姬蜂胚胎附近检测到了相同大小的细胞。检测到的细胞核数量表明浆膜由约450±150个细胞组成。在被寄生的宿主——苹果小卷蛾（Acrolepiosis assectella）中，寄生蜂卵孵化后，这些细胞在寄生蜂幼虫发育过程中并未生长。使用美丽潜蛾姬蜂卫星DNA或核糖体基因作为探针进行的Southern印迹实验表明，被寄生宿主的体内存在源自黄蜂的自由漂浮细胞。这是首次在姬蜂科物种中描述自由漂浮的类畸形细胞。
