Steiner B, Pfister-Wilhelm R, Grossniklaus-Bürgin C, Rembold H, Treiblmayr K, Lanzrein B
Division of Developmental Biology, Zoological Institute, University of Berne, Baltzerstrasse 4, CH-3012, Berne, Switzerland
J Insect Physiol. 1999 Apr;45(4):401-413. doi: 10.1016/s0022-1910(98)00139-5.
Physico-chemical analysis of juvenile hormones (JHs) of Spodoptera littoralis revealed highest quantities in the second half of embryonic development and in newly hatched 1st instar larvae. At these stages, mostly JH II, JH I and little JH III were found, while in later stages only JH II and JH III were found. Titres fluctuated in a similar manner in all larval instars, being lowest during the moults. In last (=6th) instar larvae, JHs disappeared in the late feeding-digging stage and again increased in the early prepupal stage. Parasitisation with Chelonus inanitus, a solitary egg-larval parasitoid which induces in its host the precocious onset of metamorphosis in the 5th instar, did not alter JH homologue composition but led to a disappearance of JHs in the 5th instar. Implantation of a parasitoid larva into early 5th instar larvae containing polydnavirus/venom caused a drop in the JH titre which indicates that the parasitoid larva plays an important role in the manipulation of the host's JH titre. In the parasitoid larva, only JH III was found; titres were highest in the 2nd larval instar, a stage when the host is in the 5th instar and contains almost no JHs. Thus, JHs of the parasitoid and the host fluctuate in an independent manner.
对埃及棉铃虫保幼激素（JHs）的理化分析表明，在胚胎发育后半期和新孵化的1龄幼虫中含量最高。在这些阶段，主要发现了JH II、JH I和少量的JH III，而在后期阶段只发现了JH II和JH III。在所有幼虫龄期，滴度以类似方式波动，在蜕皮期间最低。在最后（即第6）龄幼虫中，保幼激素在取食-挖掘后期消失，并在化蛹前期再次增加。用单寄生卵-幼虫寄生蜂伊氏赘寄蝇进行寄生，该寄生蜂会诱导其宿主在第5龄期早熟变态，这并没有改变保幼激素同系物的组成，但导致第5龄期保幼激素消失。将寄生蜂幼虫植入含有多角体病毒/毒液的第5龄初期幼虫中，会导致保幼激素滴度下降，这表明寄生蜂幼虫在操纵宿主保幼激素滴度方面发挥着重要作用。在寄生蜂幼虫中，只发现了JH III；滴度在第2龄幼虫期最高，此时宿主处于第5龄期，几乎不含保幼激素。因此，寄生蜂和宿主的保幼激素以独立的方式波动。
