胆红素诱导人神经母细胞瘤细胞系SH-SY5Y凋亡并影响线粒体膜电位
[Bilirubin induced apoptosis of human neuroblastoma cell line SH-SY5Y and affected the mitochondrial membrane potential].
作者信息
Han Zhang, Hu Ping, Ni Daofeng
机构信息
Department of Otorhinolaryngology, Peking Union Medical College Hospital, PUMC & CAMS, Beijing 100730, China.
出版信息
Zhonghua Er Bi Yan Hou Ke Za Zhi. 2002 Aug;37(4):243-6.
OBJECTIVE
To study whether bilirubin induces apoptosis of human neuroblastoma cell line SH-SY5Y and affects the mitochondrial membrane potential.
METHODS
SH-SY5Y cells were exposed to bilirubin, then morphological changes of nucleus were assayed by Hoechst 33258 staining. The percentage of apoptotic cells was measured by flow cytometry. The mitochondrial membrance potential(MMP) was detected by flow cytometry through Rhodamine123 staining.
RESULTS
MMP of SH-SY5Y cells significantly decreased from (16.6 +/- 1.556) U to (7.11 +/- 0.701) U (P < 0.01) after exposed to 0.02 g/L bilirubin for 1 h. The percentage of apoptotic cells was 19.4% after exposed for 3 h, and significantly increased to 76.4% after 4 h, but typical apoptotic nuclear changes occured after 6 h.
CONCLUSION
Bilirubin could early cause disruption of MMP, then induce the apoptosis of SH-SY5Y cells.
目的
研究胆红素是否诱导人神经母细胞瘤细胞系SH-SY5Y凋亡并影响线粒体膜电位。
方法
将SH-SY5Y细胞暴露于胆红素，然后用Hoechst 33258染色检测细胞核的形态变化。通过流式细胞术测量凋亡细胞的百分比。通过罗丹明123染色，用流式细胞术检测线粒体膜电位（MMP）。
结果
在暴露于0.02 g/L胆红素1小时后，SH-SY5Y细胞的MMP从（16.6±1.556）U显著降低至（7.11±0.701）U（P<0.01）。暴露3小时后凋亡细胞的百分比为19.4%，4小时后显著增加至76.4%，但6小时后出现典型的凋亡核变化。
结论
胆红素可早期导致MMP破坏，进而诱导SH-SY5Y细胞凋亡。
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