Guo Changkai, Kong Weijia
Department of Otorhinolaryngology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Zhonghua Er Bi Yan Hou Ke Za Zhi. 2002 Aug;37(4):307-9.
To study the feasibility of application of rat-tail collagen in patch clamp research in vestibular hair cells.
The effect of self-made rat-tail collagen on promoting adhesion of vestibular hair cells in whole cell patch clamp experiment was observed.
A seal was hard to be formed when the vestibular hair cells suspended among the external solution without rat-tail collagen. However, when the vestibular hair cells were firmly adhesive to the bottom of the recording chamber with rat-tail collagen, a seal can be formed easily, which fitted to the long-term observation and recording. The effect of rat-tail collagen on adhesive to vestibular hair cells is obvious.
Rat-tail collagen could facilitate the vestibular hair cells to adhesive with the bottom of the recording chamber, which is helpful for long-term patch clamp research, and the collagen is considered as an optimal adhesive reagent to vestibular hair cells for patch clamp research.
研究鼠尾胶原在前庭毛细胞膜片钳研究中的应用可行性。
在全细胞膜片钳实验中观察自制鼠尾胶原对促进前庭毛细胞黏附的作用。
在前庭毛细胞悬浮于不含鼠尾胶原的外部溶液中时，难以形成封接。然而，当用鼠尾胶原使前庭毛细胞牢固黏附于记录槽底部时，能容易地形成封接，适合进行长期观察和记录。鼠尾胶原对前庭毛细胞的黏附作用明显。
鼠尾胶原可促进前庭毛细胞与记录槽底部黏附，有助于进行长期膜片钳研究，该胶原被认为是用于膜片钳研究的前庭毛细胞的最佳黏附试剂。
