Syeda F, Senault C, Delplanque B, Le Roy B, Thaminy A, Gripois D, Blouquit M F, Ruelland A, Mendy F, Lutton C
Laboratoire de Physiologie de la Nutrition, Bât: 447, Université Paris Sud, 91405 Orsay, France.
Nutr Metab Cardiovasc Dis. 2003 Feb;13(1):28-36. doi: 10.1016/s0939-4753(03)80165-5.
Plasma cholesterol efflux capacity is stimulated during postprandial (PP) hypertriglycerdemia. Plasma cholesteryl ester transfer protein (CETP) and phospholipid transfer protein (PLTP) are the key proteins in lipoprotein metabolism and remodelling, but their role during the PP cholesterol efflux process remains indeterminate. The aim of this study was to determine the effect of a fatty meal intake on plasma CETP and PLTP activities, and the capacity of plasma to promote cholesterol efflux, as well as to evaluate the relationship between these three key mechanisms of the reverse cholesterol transport process.
CETP and PLTP activities and the cholesterol efflux capacity of plasma were measured over eight hours following a fatty meal (1000 kcal, 62% fat) in 13 normolipidemic men. CETP activity and the cholesterol efflux capacity of plasma from Fu5AH cells increased after the meal, reaching a maximum after eight hours (respectively 32%, p = 0.06, and 6.5%, p = 0.045), whereas PLTP activity remained unchanged. CETP and PLTP activities did not correlate with plasma cholesterol efflux capacity in the fasting or PP state. Plasma CETP activity in the fasting state positively correlated with the plasma non-esterified fatty acid (NEFA) levels, but no correlation was found with any lipid or apolipoprotein postprandially. The cholesterol efflux capacity of plasma correlated positively with high-density lipoprotein (HDL) components, the best correlation being with the HDL phospholipid fraction in both the fasting and PP states.
These findings suggest that plasma CETP and PLTP activities in healthy normolipidemic subjects are differently regulated in the PP state, and are not correlated with the increased cholesterol efflux capacity of PP plasma. HDL-phospholipid remains the key factor in the regulation of the capacity of plasma to promote Fu5AH cell cholesterol efflux.
餐后（PP）高甘油三酯血症期间血浆胆固醇流出能力会受到刺激。血浆胆固醇酯转运蛋白（CETP）和磷脂转运蛋白（PLTP）是脂蛋白代谢和重塑的关键蛋白，但其在PP胆固醇流出过程中的作用仍不明确。本研究的目的是确定摄入高脂餐对血浆CETP和PLTP活性以及血浆促进胆固醇流出能力的影响，并评估这三种逆向胆固醇转运过程关键机制之间的关系。
在13名血脂正常的男性摄入高脂餐（1000千卡，62%脂肪）后的8小时内，测量其CETP和PLTP活性以及血浆胆固醇流出能力。餐后，Fu5AH细胞来源的血浆中CETP活性和胆固醇流出能力增加，8小时后达到峰值（分别增加32%，p = 0.06；增加6.5%，p = 0.045），而PLTP活性保持不变。空腹或PP状态下，CETP和PLTP活性与血浆胆固醇流出能力均无相关性。空腹状态下血浆CETP活性与血浆非酯化脂肪酸（NEFA）水平呈正相关，但餐后与任何脂质或载脂蛋白均无相关性。血浆胆固醇流出能力与高密度脂蛋白（HDL）成分呈正相关，在空腹和PP状态下与HDL磷脂部分的相关性最佳。
这些发现表明，健康血脂正常受试者血浆中的CETP和PLTP活性在PP状态下受到不同调节，且与PP血浆中增加的胆固醇流出能力无关。HDL-磷脂仍然是调节血浆促进Fu5AH细胞胆固醇流出能力的关键因素。
