Ferýha Sussanne, Ercan Feriha, Arbak Serap
Department of Histology-Embryology, School of Medicine, Marmara University, Haydarpaşa, Istanbul, Turkey.
Dig Dis Sci. 2003 May;48(5):1006-12. doi: 10.1023/a:1023028402822.
The increasing use of the small bowel for transplantation necessitates the development of optimum preservation techniques. The aim of our study was to investigate time-related morphological changes of the rat small bowel during preservation of the organ in hypothermic Euro-Collins and Lactated Ringer's solutions. It is the only study comparing these widely used preservation solutions with scanning electron microscopy, which is especially suitable to show the intestinal mucosa, one of the tissues of the intestine most susceptible to ischemia. Small bowels were perfused with either Euro-Collins, Lactated Ringer's or physiological saline solution and placed in respective preservation solutions at 4 degrees C for 0, 3, 6 and 12 hours. Biopsy samples were prepared for examination at the light and scanning electron microscopic levels. According to the results of this study we conclude that, even Lactated Ringer's solution showed better tissue preservation than Euro-Collins solution, both preservation solutions are suitable for short term preservation of the small bowel and that further ameliorations of the preservation solutions and/or techniques are required for long term preservation.
小肠移植应用的日益增加使得开发最佳保存技术成为必要。我们研究的目的是调查大鼠小肠在冷缺血状态下于欧洲柯林斯液和乳酸林格液中保存期间与时间相关的形态学变化。这是唯一一项通过扫描电子显微镜比较这两种广泛使用的保存液的研究，扫描电子显微镜特别适合显示肠黏膜，它是肠道中对缺血最敏感的组织之一。将小肠分别用欧洲柯林斯液、乳酸林格液或生理盐水灌注，然后置于相应的保存液中，在4℃下保存0、3、6和12小时。制备活检样本用于光镜和扫描电镜检查。根据本研究结果，我们得出结论：即使乳酸林格液显示出比欧洲柯林斯液更好的组织保存效果，但两种保存液都适用于小肠的短期保存，而长期保存则需要对保存液和/或技术进行进一步改进。
