Peng Ming-li, Ren Hong, Xu Hong-mei, Chen Min, Ling Ning, Qin Yu-ling
Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010, China.
Zhonghua Gan Zang Bing Za Zhi. 2003 May;11(5):271-4.
To explore the possibility of cell-medicated immune response induced with heat shock protein 70 (HSP70)-HBsAg protein complex in vitro.
HSP70-HBsAg complex was reconstituted in vitro which was injected into mice in order to observe that whether HSP70-HBsAg would stimulate humoral and cellular immune responses. HSP70, HSP70-HBsAg complex and HBsAg were used to activate the dentritic cell (DC) individually, which would initiate homogeneic T lymphocyte to transform to cytotoxic T lymphocyte (CTL). The cytotoxicity of CTL was detected with MTT assay.
HSP70-HBsAg complex elicited both humoral and cellular immune responses against HBsAg in mice. Specific CD8+ CTL response was readily induced by HSP70-HBsAg complex and HBsAg, especially the former.
HSP70-HBsAg complex is immunogenic and HSP70 can lead to great efficient CTL response. And HSP70-HBsAg complex may be used as a protein vaccine for immunotherapy for chronic hepatitis B.
探讨热休克蛋白70（HSP70）-乙肝表面抗原（HBsAg）蛋白复合物在体外诱导细胞介导免疫反应的可能性。
体外重组HSP70-HBsAg复合物并注射到小鼠体内，观察其是否能刺激体液免疫和细胞免疫反应。分别用HSP70、HSP70-HBsAg复合物和HBsAg激活树突状细胞（DC），进而启动同种异体T淋巴细胞转化为细胞毒性T淋巴细胞（CTL）。采用MTT法检测CTL的细胞毒性。
HSP70-HBsAg复合物在小鼠体内引发了针对HBsAg的体液免疫和细胞免疫反应。HSP70-HBsAg复合物和HBsAg均可诱导特异性CD8+ CTL反应，尤其是前者。
HSP70-HBsAg复合物具有免疫原性，HSP70可引发高效的CTL反应。HSP70-HBsAg复合物有望作为一种蛋白疫苗用于慢性乙型肝炎的免疫治疗。
