Spooner R J, Delides A, Goldberg D M
Clin Chem. 1976 Jul;22(7):1005-8.
Serum glutathione reductase activity decreases on standing at room temperature. Dilution of the sample slows this decay in sera from patients with a variety of clinical disorders, and reverses the inactivation in sera from patients with liver or biliary tract disease. Prolonged contact with dithioeythritol or acetate buffer, pH 6.4, at 4 degrees C prevents spontaneous inactivation of the native serum and abolishes the effect of dilution. These hitherto unreported phenomena are a source of potential inaccuracy in assays for this enzyme. The inactivation may be the result of conformational folding with "burying" of active sites, or of molecular aggregation brought about by hydrogen bonding or disulfide bond formation.
血清谷胱甘肽还原酶活性在室温下放置时会降低。样本稀释可减缓患有各种临床疾病患者血清中的这种衰减，并使肝脏或胆道疾病患者血清中的失活现象逆转。在4℃下与二硫苏糖醇或pH 6.4的乙酸盐缓冲液长时间接触可防止天然血清自发失活，并消除稀释的影响。这些迄今为止未报道的现象是该酶检测中潜在误差的来源。失活可能是活性位点“埋藏”导致的构象折叠的结果，或者是由氢键或二硫键形成引起的分子聚集的结果。
