曲匹地尔对大鼠小肠缺血/再灌注损伤的影响。

Effect of trapidil in ischemia/reperfusion injury on rat small intestine.

作者信息

Colak Tahsin, Polat Ayse, Bagdatoglu Ozlen, Kanik Arzu, Turkmenoglu Ozgur, Aydin Suha

机构信息

Department of General Surgery, Faculty of Medicine, Mersin University, Tip Fakultesi Hastanesi, Zetinlibahce C. 33079 Mersin, Turkey.

出版信息

J Invest Surg. 2003 May-Jun;16(3):167-76.

PMID:12775433

Abstract

To investigate the effect of trapidil on the intestinal ischemia-reperfusion injury, we determined malondialdehyde levels as a indicator of lipid peroxidation, nitrite and nitrate levels as reflections of nitric oxide metabolism, and histopathological findings in rats subjected to 40 min of ischemia and 2 h of reperfusion. Histopathological evaluation demonstrated that trapidil treatment has a protective effect on intestinal mucosa and reduces inflammatory cell infiltration in lamina propria, which is consistently noted in the untreated ischemic and reperfused intestines. Possible mechanism of this effect may be explained by the reduced lipid peroxidation (mean malondialdehyde level 3.72 +/- 0.27 vs. 6.13 +/- 0.44, p <.0001) and improved nitric oxide metabolism (mean nitrite plus nitrate 38.21 +/- 2.33 vs. 30.14 +/- 1.47, p =.022).

摘要

为研究曲匹地尔对肠缺血再灌注损伤的影响，我们测定了丙二醛水平（作为脂质过氧化的指标）、亚硝酸盐和硝酸盐水平（作为一氧化氮代谢的反映），并观察了经历40分钟缺血和2小时再灌注的大鼠的组织病理学表现。组织病理学评估显示，曲匹地尔治疗对肠黏膜有保护作用，并减少了固有层中的炎性细胞浸润，而在未经治疗的缺血再灌注肠中则持续存在这种情况。这种作用的可能机制可以通过脂质过氧化减少（丙二醛平均水平3.72±0.27对6.13±0.44，p<.0001）和一氧化氮代谢改善（亚硝酸盐加硝酸盐平均水平38.21±2.33对30.14±1.47，p=.022）来解释。